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pT7-EGFP-C1-HCV-RLuc-6xbsMS2-polyA-Malat_AC

Plasmid

#148448

Purpose

Mammalian Expression of 6xbsMS2-polyA-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-polyA-Malat

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2200

Plasmid

#170407

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->3] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ702-716, replace with 347-360)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2201

Plasmid

#170408

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->5] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ702-716, replace with 580-600)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2254

Plasmid

#170409

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->2] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ700-717, replace with 225-249)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2255

Plasmid

#170410

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->4] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ700-716, replace with 445-479)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2256

Plasmid

#170411

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->7] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ700-717, replace with 812-836)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2116

Plasmid

#170393

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 1 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ48-159)

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2117

Plasmid

#170394

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 2 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ160-279)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2118

Plasmid

#170395

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 3 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ280-391)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2119

Plasmid

#170396

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 4 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ392-515)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2120

Plasmid

#170397

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 5 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ516-633)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2121

Plasmid

#170398

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 6 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ634-746)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2122

Plasmid

#170399

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 7 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ747-877)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2199

Plasmid

#170406

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->1] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ702-717, replace with 114-130)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2114

Plasmid

#175961

Purpose

For expression of LetA-[ΔRing6 LetB] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Tags

8xHis 2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

Δ634-746

Promoter

araBAD

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target2 (Mpphot)

Plasmid

#186729

Purpose

Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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px459-H3.1 (Hist1h3g) sgRNA

Plasmid

#186930

Purpose

Hist1h3g sgRNA used for genomic targeting at Hist1h3g C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Hist1h3g sgRNA (H3c8 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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ppwd1.473.646.W601A

Plasmid

#137660

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

Insert

ppwd1 (PPWD1 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

473-646-W601A

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM262 - pDESTR4-R3 mosTI hygroR target

Plasmid

#159840

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI hygroR target

Expression

Worm

Mutation

Not applicable

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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Rp51a_10bp 5'SS_No BP or pBP_pUC18

Plasmid

#100989

Purpose

Rp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

tGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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