We narrowed to 22,830 results for: Sis

1. pRN227

   Plasmid

   #127222

   Purpose

   BeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Cas9, WUS2, AtSTM, sgRNA
   

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74010

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5xUAS
   Cas9
   Cre
   U6 promoter
   

   Use

   CRISPR

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFYF1327 EGFP Site#2

   Plasmid

   #47512

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF464

   Plasmid

   #167672

   Purpose

   Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. WFS1-Flag-pcDNA3

   Plasmid

   #13011

   Depositor

   Fumihiko Urano

   Article

   Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

   Insert

   Wolfram Syndrome 1 (WFS1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Sept. 26, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMH1105

   Plasmid

   #131864

   Purpose

   Produces holo-PhyB-AviTag in E. coli

   Depositor

   Wilfried Weber

   Article

   Horner et al Bio Protoc. 2020 Mar 5;10(5):e3541. doi:

   Inserts

   PhyB(1-651)-AviTag-His6
   HO1
   PcyA
   

   Expression

   Bacterial

   Promoter

   T7 promoter-1 and T7 promoter-2

   Available Since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe-vHras-zeo

   Plasmid

   #17756

   Depositor

   Susan Lindquist

   Article

   Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

   Insert

   vHras (genomic0 (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   V12D

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pSF463

   Plasmid

   #167671

   Purpose

   Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. aP2-luciferase

   Plasmid

   #8858

   Depositor

   Bruce Spiegelman

   Article

   Graves et al Mol Cell Biol. 1992 Mar . 12(3):1202-8.

   Insert

   aP2 enhancer / promoter (Fabp4 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase reporter

   Expression

   Mammalian

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-SEP_NR2A

    Plasmid

    #23997

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    NR2A (Grin2a Rat)
    

    Tags

    SEP

    Expression

    Mammalian

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-AAV5cap

    Plasmid

    #198015

    Purpose

    For high-level expression of the AAV5 cap gene, suitable for use with pCMV-Rep78/68 SSM library

    Depositor

    George Church

    Article

    Jain et al Elife. 2024 Mar 27;12:RP87730. doi: 10.7

    Insert

    AAV5 cap
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-N1-delta30-TFEB

    Plasmid

    #44445

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFEB (TFEB Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-30; H32D

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_CMV:Flag-NLuc_SV40:PuroR

    Plasmid

    #183045

    Purpose

    Expresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    NanoLuciferase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. LbABE8e

    Plasmid

    #138504

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-dLbCas12a
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMF-FU_TetO_GW_PPARG2

    Plasmid

    #193289

    Purpose

    Lentiviral construct that expresses a dox-inducible human PPARG2

    Depositor

    Rudolf Jaenisch

    Article

    Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

    Insert

    PPARG2 (PPARG2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. hGFAP-NLS-HA-Dre-P2A-BFP

    Plasmid

    #51277

    Purpose

    Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS-HA-Dre-P2A-BFP
    

    Expression

    Mammalian

    Promoter

    hGFAP

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEos4b-C1

    Plasmid

    #54812

    Purpose

    Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson, Loren Looger

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Tags

    mEos4b

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. SpCas9-CBE6c

    Plasmid

    #215821

    Purpose

    Express CBE6c (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6c-SpCas9-2xUGI (cas9 )
    

    Expression

    Mammalian

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti-CMV-TFAM-BioID2-IRES-Puro

    Plasmid

    #211465

    Purpose

    Lentivirus backbone expressing human TFAM fused with BioID2

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    TFAM-BioID2
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. JD639

    Plasmid

    #160398

    Purpose

    Binary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformation

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    35S::vitis miR396-resistant GRF4-GIF1 chimera
    

    Use

    Binary vector

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMaCTag-Z13

    Plasmid

    #120056

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry
    

    Use

    Pcr template

    Tags

    mCherry

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. TLCV2-sgHPDL #3

    Plasmid

    #174165

    Purpose

    knock out HPDL in human cell lines

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    sgRNA against HPDL
    

    Use

    Lentiviral

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. TLCV2-sgHPDL #9

    Plasmid

    #174166

    Purpose

    knock out HPDL in human cell lines

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    sgRNA against HPDL
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCPP6534

    Plasmid

    #128735

    Purpose

    Gateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal HA tag

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CAV1-mEGFP

    Plasmid

    #27704

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Available Since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p6659 MSCV-IP N FlagHA HPV16E6

    Plasmid

    #44152

    Depositor

    Peter Howley

    Article

    White et al J Virol. 2012 Dec;86(24):13174-86. doi:

    Insert

    HPV16E6
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pAS006

    Plasmid

    #211506

    Purpose

    AAV genome backbone with a CROP-seq-like design for indirect capture of gRNA sequences

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Type

    Empty backbone

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. CRY2high-mCherry

    Plasmid

    #104063

    Purpose

    Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2high-mCherry
    

    Expression

    Mammalian

    Mutation

    CRY2PHR E490R

    Promoter

    CMV

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI

    Plasmid

    #169097

    Purpose

    Cas9 and Citrine expression in transfected cells

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Development. 2021 Mar 9. pii: dev.193565

    Insert

    Cas9-2A-Citrine
    

    Expression

    Mammalian

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLKO.1-shCtnnd2.1-GFP

    Plasmid

    #209095

    Purpose

    GFP expressing shRNA targeting Ctnnd2 N-terminus

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCtnnd2.1
    

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSF-CjPglB

    Plasmid

    #199305

    Purpose

    pSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    CjPglB with flag tag
    

    Tags

    Flag tag

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMaCTag-Z07

    Plasmid

    #124790

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeonGreen
    

    Use

    Pcr template

    Tags

    mNeonGreen

    Promoter

    None

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLX317 GFP

    Plasmid

    #193681

    Purpose

    Constitutive lentiviral expression of GFP

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    GFP
    

    Use

    Lentiviral

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGreen_dualluc_3'UTR_sensor

    Plasmid

    #55206

    Purpose

    Dual-luciferase reporter for microRNA targeting in plants

    Depositor

    Michael Axtell

    Article

    Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

    Inserts

    Renilla luciferase
    Firefly luciferase
    

    Use

    Luciferase; Plant transient expression

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBAD24-sfGFPx2

    Plasmid

    #51559

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

    Depositor

    Sankar Adhya, Francisco Malagon

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Tags

    GLESTCRHASLAVLADERRFSA and MARARA

    Expression

    Bacterial

    Mutation

    Contains additonal aa in the N and C terminus (in…

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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36. pLJC2-Blast-SHMT2-3xFLAG

    Plasmid

    #233891

    Purpose

    lentiviral overexpression vector for SHMT2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

    Insert

    SHMT2 (SHMT2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDual-eGFP

    Plasmid

    #63215

    Purpose

    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pCMV Flag WT-PTEN

    Plasmid

    #22231

    Depositor

    Hong Wu

    Article

    Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

    Insert

    PTEN (PTEN Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBMPC3

    Plasmid

    #31367

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Bmal1 (Bmal1 Mouse)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CRY2low-tdTomato

    Plasmid

    #104067

    Purpose

    Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomato

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2low-tdTomato
    

    Expression

    Mammalian

    Mutation

    CRY2 aa 1-491 truncation, R489E A491D

    Promoter

    CMV

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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