We narrowed to 18,311 results for: puro
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Plasmid#154774Purposeplasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
TagsFLAGExpressionMammalianMutationin PylT: UUA anticodon and G10A, G14U, G16U, C20U…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX Nup62Fv2GFP
Plasmid#139683PurposeNup62 dimerization construct fused to 2 copies of GFP under an CMV promoterDepositorInsertNup62 (NUP62 Human)
UseLentiviralTags2 copies of GFP and Dimerization domain FKBPExpressionMammalianPromoterCMVAvailable SinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-CDK12_IDG-K
Plasmid#135281PurposeGateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-CDK12 (CDK12 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TBK1-C-FLAG-HA
Plasmid#131792PurposeLentiviral vector expressing WT TBK1 containing STOP codonDepositorInsertTBK1 WT (TBK1 Human)
UseLentiviralTagsSTOP codonExpressionMammalianMutationWT full lengthPromoterCMVAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rInsp-hIns-GLuc
Plasmid#89927PurposeExpresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
UseLentiviralExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…Promoter410bp rat insulin II promoterAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-NLS-TIR1_P2A_NES-TIR1
Plasmid#117699PurposeExpression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNADepositorInsertsTRANSPORT INHIBITOR RESPONSE 1
TRANSPORT INHIBITOR RESPONSE 1
TagsHA tag, Myc-NES, and NLSExpressionMammalianMutationcodon optimized for murine expressionPromoterNone, 2A peptide from porcine teschovirus-1 polyp…Available SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP 3xFLAG-VMP1
Plasmid#117416PurposeExpresses VMP1 tagged with 3xFLAG in mammalian cellsDepositorAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-EGFP:Ajuba
Plasmid#108229PurposeLentiviral vector for Tet-inducible Ajuba expressionDepositorInsertAJUBA (AJUBA Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterTet/ON Minimal CMV promoterAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
HNF1B_pLX307
Plasmid#98342PurposeLentiviral expression of HNF1BDepositorAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
IQGAP1_pLX307
Plasmid#98346PurposeLentiviral expression of IQGAP1DepositorAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
BRCA1 A10.2 gRNA
Plasmid#90549Purpose3rd generation lentiviral gRNA plasmid targeting human BRCA1DepositorInsertBRCA1 (Guide Designation A10.2)
UseCRISPR and LentiviralPromoterU6Available SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
ires-eGFP(+HAs) donor-1
Plasmid#87865PurposeExpress eGFP reporter for HDR-induced knock-inDepositorInserteGFP
UseHomologous recombination for gapdh lociAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ (M)-HT-Cbx4
Plasmid#82513PurposeLentivirus vector. Expresses Halotag-Cbx4 fusion proteins in mammalian cells.DepositorInsertChromobox Homolog 4 (Cbx4 Mouse)
UseLentiviralTagsHaloTagExpressionMammalianPromotercmvAvailable SinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA2-gRNA_A
Plasmid#74376PurposegRNA_A to knockout human AMPK alpha 2 using Cas9n.DepositorAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-INHBA
Plasmid#73015PurposeRetroviral expression of human activin ADepositorAvailable SinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP/SECFP-hATG9A
Plasmid#58995PurposeExpresses SECFP-hATG9A in mammalian cellsDepositorAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
TGFbetaR1 (T204D)-pcw107-V5
Plasmid#64629Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertTGFBR1 (transcript variant 1) (TGFBR1 Human)
UseLentiviralTagsV5MutationT204DPromoterPGKAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (Sce)
Plasmid#45576PurposeCodes for the SSA-TLR with the target site for I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Sce I TS
+3 mCherry
3' iRFP arm
UseLentiviralExpressionBacterial and MammalianMutationembedded I-Sce I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
p6659 MSCV-IP N FlagHA HPV16E6
Plasmid#44152DepositorInsertHPV16E6
UseRetroviralTagsFlag and HAExpressionMammalianPromoterMSCV LTRAvailable SinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP-EGFP-ULK1(K46N)
Plasmid#38197DepositorInserta novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationchanged Lysine 46 to AsparagineAvailable SinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only