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UAS-LA::Halo2

Plasmid

#87651

Purpose

For creating drosophila transgenics expressing UAS-LA::Halo2

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

LA::Halo2

Expression

Insect

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pattB-RfB2

Plasmid

#62296

Purpose

docking site transgenesis plasmid for Gateway Cloning and insect transgenesis

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Unspecified

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pWJPrP43

Plasmid

#22112

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

PrP ki-3F4-FFI TC

Use

Mouse Targeting; Construction of targeting vector

Mutation

3F4 epitope and FFI related D177N mutation

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
sCAG-DIO-oScarlet3

Plasmid

#247721

Purpose

Used for AAV1 tracing experiment.

Depositor

Boxuan Zhao

Article

Chen et al bioRxiv [Preprint]. 2025 May 26:2025.02.

Insert

oScarlet3

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist_21tRNAs_split

Plasmid

#246822

Purpose

Plasmid for simultaneous expression of the minimum 21 tRNA set

Depositor

Norikazu Ichihashi

Article

Miyachi et al Nat Commun. 2025 Aug 26;16(1):7418. doi:

Insert

E. coli tRNA arrays

Expression

Bacterial

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET-C5

Plasmid

#246823

Purpose

Expression plasmid for C5 protein of E. coli RNase P

Depositor

Norikazu Ichihashi, Takashi Yokogawa

Article

Miyachi et al Nat Commun. 2025 Aug 26;16(1):7418. doi:

Insert

C5 protein of E. coli RNase P

Tags

Histidine tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5427

Plasmid

#229034

Purpose

tetO-phi29 DNA polymerase ‘dead’ reporter: PT7-tetO-B0035-p2 D249E-T7 terminator, AmpR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-tetO-RBS35-phi29 DNAP (D249E, dead)-T7term

Expression

Bacterial

Promoter

PT7-tetO

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5451

Plasmid

#231066

Purpose

tetO-10xHis-phi29 DNA polymerase PovSak reporter: PT7-tetO-B0035-10xHis-p2 povsak-T7 terminator, AmpR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-tetO-B0035-10xHis-phi29 DNAP PovSak-T7term

Expression

Bacterial

Promoter

PT7-tetO

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_CKAR3

Plasmid

#238411

Purpose

Mammalian expression of CKAR3 under a CMV promotor.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

CKAR3 (PKC sensor)

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mutCKAR3

Plasmid

#238412

Purpose

Mammalian expression of a phosphorylation deficient (T to A) mutant of CKAR3 under a CMV promotor.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

phosphorylation deficient mutant of CKAR3 (T to A)

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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MKT074

Plasmid

#231997

Purpose

MicF RNA toehold switch with NanoLuc complementation peptide reporter

Depositor

Melissa Takahashi

Article

Diaz et al MicroPubl Biol. 2025 Jan 2;2025:10.17912

Insert

MicF RNA toehold switch

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pUlaR

Plasmid

#216327

Purpose

T7 promoter driving expression of ulaR

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

L-Ascorbate-6-P-responsive transciptional repressor

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBad_UlaABC

Plasmid

#216328

Purpose

Arabinose-inducible PBad promoter driving expression of ulaA, ulaB, and ulaC

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

UlaABC

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAM254

Plasmid

#226898

Purpose

mEOS3.2-IDR*-(7aaSG)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Tags

7aaSG Prescission GSS-FLAG-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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MAP80A_MS2

Plasmid

#220624

Purpose

tRNA reporter vector

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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KNtp-SUR1 core fusion

Plasmid

#216212

Purpose

for expression of KNtp-SUR1 fusion protein in BacMam system

Depositor

Lei Chen

Article

Wang et al Front Pharmacol. 2022 Jun 30;13:929684.

Insert

SUR1 core

Tags

GFP-MBP

Expression

Mammalian

Mutation

deletion of TMD0

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pH2B-6AA-ffDronpa

Plasmid

#214606

Purpose

Expresses reversibly switchable fluorescent protein ffDronpa targeted to the nucleus

Depositor

Peter Dedecker

Article

Valenta et al Talanta. 2024 Mar

Insert

ffDronpa-H2B

Tags

H2B targetting with a linker of 6AA

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pffDronpa2F-peroxisome

Plasmid

#214608

Purpose

Expresses reversibly switchable fluorescent protein ffDronpa2F targeted to peroxisome

Depositor

Peter Dedecker

Article

Valenta et al Talanta. 2024 Mar

Insert

ffDronpa2F-peroxisome

Tags

peroxisome targeting sequence (PTS)

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-neo C-HAonly HPV16 E7 E80A/D81A

Plasmid

#191541

Purpose

Protein expression of HPV16 E7 E80A/D81A

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

HPV16 E7

Use

Retroviral

Tags

HA

Mutation

E80A, D81A

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAFNF-EGFP

Plasmid

#163609

Purpose

Expresses EGFP Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Egfp

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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