We narrowed to 8,039 results for: Dos;

1. pLJC6-2XFLAG-TMEM192

   Plasmid

   #104435

   Purpose

   Expresses 2XFLAG-TMEM192 in mammalian cells

   Depositor

   David Sabatini

   Article

   Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

   Insert

   TMEM-192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   2XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIDS-RESTwt full-length

   Plasmid

   #109161

   Purpose

   Encodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Inui et al Protein Sci. 2017 May;26(5):997-1011. do

   Insert

   human full-length REST/NRSF (REST Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   p10

   Available since

   Jan. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-HisD-mNG-Ec-Kbp-D9N

   Plasmid

   #178011

   Purpose

   Bacterial expression of green fluorescent potassium indicator mNG-Ec-Kbp-D9N (KRaION1 mutant D9N)

   Depositor

   Edward Boyden

   Article

   Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

   Insert

   mNG-Ec-Kbp-D9N
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araB

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304 3xFlag-eRF1_IRES-GFP

   Plasmid

   #198393

   Purpose

   eRF1 expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   eRF1 (ETF1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEYFP-N1-Pink1

   Plasmid

   #101874

   Purpose

   Transient expression of Pink1-YFP in mammalian cells

   Depositor

   Richard Youle

   Article

   Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

   Insert

   PTEN induced putative kinase 1 (PINK1 Human)
   

   Use

   Tags

   fluorescent tag EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-Cas9-6xHis

   Plasmid

   #62374

   Purpose

   Expression of Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9_6xHis
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Cas9-NLS-6xHis

   Plasmid

   #62933

   Purpose

   Expression of Cas9-NLS-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9-NLS-6xHis
   

   Use

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-BAF

   Plasmid

   #101772

   Purpose

   Expresses EGFP tagged BAF in human cells

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Gβ-2A-cpV-Gγ2-IRES-Gαi2-mTq2

   Plasmid

   #69624

   Purpose

   A FRET sensor for Galphai2 activation, encoded on a single plasmid.

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Inserts

   Gbeta-2A-cpV-Ggamma2
   Galphai2-mTurquoise2
   

   Use

   Tags

   ciruclar permutated Venus (cp173V) and mTurquoise…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and CMV (IRES)

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDG461

    Plasmid

    #100902

    Purpose

    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. tph-1(short)::rpl-22-HA

    Plasmid

    #122203

    Purpose

    C. elegans tagged ribosome expression plasmid

    Depositor

    Steven Flavell

    Article

    Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

    Insert

    tph-1(short)::rpl-22-HA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_YWHAG_14-3-3

    Plasmid

    #109905

    Purpose

    Protein expression and purification of YWHAG_14-3-3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    YWHAG_14-3-3 (YWHAG Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-LaM-4

    Plasmid

    #172770

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-4
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. FZ-NbPDS

    Plasmid

    #184268

    Purpose

    pAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.

    Depositor

    Feng Qu

    Article

    Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do

    Insert

    CPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    The protease processing site between MP and L-CP …

    Promoter

    CaMV 35S with duplicated enhancer

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Frankenbody-FLAG-Halo

    Plasmid

    #175296

    Purpose

    Track mature and nascent FLAG tagged proteins in live cells

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-FLAG frankenbody fused with HaloTag
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcw107

    Plasmid

    #62511

    Purpose

    Gateway Expression Vector

    Depositor

    David Sabatini, John Doench

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-N2-Frankenbody-HA

    Plasmid

    #175292

    Purpose

    Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-HA frankenbody fused with mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183741

    Purpose

    Expresses Ki67 (8 repeats+LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67 LR domain + 8 Ki-67 repeats

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2

    Plasmid

    #69623

    Purpose

    A FRET sensor for Galphai1 activation, encoded on a single plasmid.

    Depositor

    Dorus Gadella

    Article

    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

    Inserts

    Gbeta-2A-cpV-Ggamma2
    Galphai-mTurquoise2
    

    Use

    Tags

    ciruclar permutated Venus (cp173V) and mTurquoise…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and CMV (IRES)

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPPED

    Plasmid

    #162468

    Purpose

    Destination Expression vector for dicot plant prime editing

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Inserts

    Cas9H840A
    attR gateway destination cassette
    

    Use

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine based sequence o…

    Promoter

    2x35S

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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