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T7-VEE-IRES-Puro

Plasmid

#58970

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pTNT-B18R-6His

Plasmid

#58979

Purpose

expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Tags

His

Expression

Bacterial

Promoter

T7/Sp6

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-Thy1.1 DEST

Plasmid

#17442

Purpose

Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pUC19-eGFP-P2A-dTAG-Ing3

Plasmid

#170754

Purpose

Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

Depositor

Kristian Helin

Article

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

eGFP-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

eGFP-P2A-2xHA-dTAG

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

none

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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p2xp53_RE

Plasmid

#118053

Purpose

GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

2 copies of the p53 DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00552

Plasmid

#39647

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10699 (Lim3 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00091

Plasmid

#34247

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1071 (E2f2 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pBa.HALO-KIF3A tail 419-702

Plasmid

#193924

Purpose

Mammalian expression of Halo-tagged Kif 3A tail 419-702

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Tags

Halo

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KIF3B tail 391-747

Plasmid

#193929

Purpose

Mammalian expression of Halo-tagged Kif 3B tail 391-747

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3B (Kif3b Mouse)

Tags

Halo

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3A tail 419-702

Plasmid

#193923

Purpose

Mammalian expression of GFP-tagged Kif 3A tail 419-702

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pKM28

Plasmid

#154165

Purpose

Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Rel1A

Tags

FLAG

Expression

Insect

Promoter

IE1 promoter and hr5 enhancer from Autographa cal…

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00217

Plasmid

#34366

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG4158 (wor Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00171

Plasmid

#34324

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10619 (tup Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-Egr1

Plasmid

#11729

Depositor

Eileen Adamson

Article

Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

Insert

Egr-1 (Egr1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pNANOG-Luc

Plasmid

#25900

Depositor

Ren-he Xu

Article

Xu et al Cell Stem Cell. 2008 Aug 7. 3(2):196-206

Insert

NANOG promoter (NANOG Human)

Tags

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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KA1396_pGL4-UBCmin-luc2/Klf4-E'

Plasmid

#124187

Purpose

Luciferase reporter driven by the Klf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf4 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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KA2626_pGL4-UBCmin-luc2/Klf2-D'

Plasmid

#124199

Purpose

Luciferase reporter driven by the Klf2 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf2 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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KA1401_pGL4-UBCmin-luc2/Klf4-F'

Plasmid

#124188

Purpose

Luciferase reporter driven by the Klf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf4 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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KA1395_pGL4-UBCmin-luc2/Klf4-B'

Plasmid

#124186

Purpose

Luciferase reporter driven by the Klf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf4 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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SP6-VEE-IRES-Puro

Plasmid

#58971

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge