We narrowed to 8,640 results for: dykddddk
-
Plasmid#106275PurposeEncodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEcoCasE-p300
UseCRISPRTagsE tag, NLS and FlagExpressionMammalianPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAD108_Arg273His_pCMV6Entry-hIRG1
Plasmid#124878PurposeExpresses hCAD Arg273His mutant in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcis-aconitate decarboxylase (ACOD1 Human)
Tagsmyc and FLAG tagsExpressionMammalianMutationmutated Arg273 to HisPromoterCMVAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
MigR1-Flag-TRIB2
Plasmid#131154PurposeRetroviral expression of flag-tagged mouse TRIB2DepositorAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA6-N-3XFLAG-Fzd6
Plasmid#123588DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertfrizzled class receptor 6 (Fzd6 Mouse)
TagsFLAGExpressionMammalianAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-UBE2G1 sg2R-P2A-Hygro
Plasmid#124299PurposeLentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.DepositorInsertUBE2G1 (UBE2G1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationseveral silent mutations to disrupt sgRNA targeti…PromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q85
Plasmid#111736PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ85 (polyQ repeat)PromoterCMV and P10Available sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-E31A
Plasmid#125843Purposeexpress mutant BAP1-E31ADepositorAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-S10T
Plasmid#125842Purposeexpress mutant BAP1-S10TDepositorAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG membrane-bound lipin-1 (TMD)
Plasmid#120277PurposeExpression of ER/Golgi-tethered lipin-1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLipin-1 (Lpin1 Mouse)
TagsFLAG and in-frame fusion with the TMD Golgi-local…ExpressionMammalianPromoterCMVAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR
Plasmid#113162PurposeA donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable markerDepositorInsertSpCas9
TagsFLAGExpressionMammalianMutationD10APromoterCAGAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 ECD-MHC
Plasmid#113957Purposemammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class IDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG, Signal/leader sequence from influenza hemag…ExpressionMammalianMutationresidues 22-568 fused to a transmembrane tetherPromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113688PurposeSaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.DepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSPromoterCytomegalo Virus(CMV)Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113689PurposeSaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACSDepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSPromoterCytomegalo Virus(CMV)Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUW-hTie1-FLAG
Plasmid#119233PurposePlasmid encodes FLAG-tagged full-length human Tie1 gene cloned in lentiviral vector.DepositorAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-SFXN5
Plasmid#110639PurposeRetroviral expression of Flag-SFXN5 in mammalian cellsDepositorAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/Flag-mKO2
Plasmid#110371PurposeMammalian expression of mKO2 with FLAG tag, Flp-In T-Rex systemDepositorInsertmonomeric Kusabira Orange 2
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73
Plasmid#111724PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ73 (polyQ repeat)PromoterCMV and P10Available sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag RagB(GD)/RagC(CTD) chimera
Plasmid#112773Purposeexpress Flag-RagB (Gdomain) fused with RagC (C terminal domain)DepositorInsertFlag-RagB (G domain) fused with RagC (C terminal domain) (RRAGC Human)
TagsFlagExpressionMammalianAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
CelTOS-COMP-blac-flag-his
Plasmid#111014PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertcell traversal protein for ookinetes and sporozoites (CelTOS) (PF3D7_1216600 )
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
GRP94-COMP-blac-flag-his
Plasmid#111008PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertendoplasmin, putative (GRP94) (PFL1070c Synthetic)
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only