We narrowed to 5,560 results for: Chia

1. ABE-RA2.0

   Plasmid

   #208299

   Purpose

   Expresses ABE-RA2.0 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA2.0-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ABE-RA3.1

   Plasmid

   #208302

   Purpose

   Expresses ABE-RA3.1 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA3.1-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBES1-LacOx50-blast

   Plasmid

   #210221

   Purpose

   Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   50xLacO repeats derived from: PMID: 12864855.
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pG108 - K

   Plasmid

   #178039

   Purpose

   Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

   Depositor

   Janina Lewis

   Article

   Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

   Insert

   Kanamycin resistance
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ErmAM has been replaced with Kanamycin for select…

   Promoter

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MscL(G22S)-c-term-cpGFP-ERexp

   Plasmid

   #195346

   Purpose

   Control plasmid for the MscL tension biosensor

   Depositor

   Allen Liu

   Article

   Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

   Insert

   MscL(G22S)-c-term-cpGFP-ERexp
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVd2

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 FLIPglu-600u

   Plasmid

   #13572

   Depositor

   Wolf Frommer

   Article

   Fehr et al J Biol Chem. 2003 May 23. 278(21):19127-

   Insert

   FLIPglu F16A
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Mammalian

   Mutation

   F16A in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-mNeon-TurboID

   Plasmid

   #186610

   Purpose

   Third generation lentiviral vector expressing mNeonGreen fused to V5-TurboID

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   mNeon-V5-TurboID
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   For reference see Branon et al., 2018

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET FLIPE-600n

   Plasmid

   #13537

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E WT
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEvoltac EcW-TGA-h9

   Plasmid

   #218765

   Purpose

   encodes EcWRS-h9 and EcW-tRNA-TGA

   Depositor

   Abhishek Chatterjee

   Article

   Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

   Insert

   EcWRS-h9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S8A, V144S, V146A

   Promoter

   tacI

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTuLys2CMR2

    Plasmid

    #53544

    Purpose

    Inhibition module. Constructed from the parent plasmids pLuxRI, pLuxmCherry, pET15bLCFPT7, and pLysS. The plasmid can express T7 lysozyme by inducing AHL and LuxR. Chloramphenicol resistance

    Depositor

    Lingchong You

    Article

    Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

    Insert

    pLux
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET303-colE1T

    Plasmid

    #155180

    Purpose

    Expresses colicin E1 residues 1-190

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    colicin E1 residues 1-190
    

    Use

    Tags

    C-terminal 6x Histidine tag

    Expression

    Bacterial

    Mutation

    truncation of colicin E1 including 1-190

    Promoter

    T7

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSF-1b-S2

    Plasmid

    #128590

    Purpose

    Expresses tagless S2 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S2 (rpsB E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPL5071_TEF1*-Cre_URA3

    Plasmid

    #60930

    Purpose

    Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHis17_ParM _1

    Plasmid

    #78201

    Purpose

    Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

    Depositor

    Martin Webb

    Article

    Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

    Insert

    parM (K33A I27C T174A T175N C287A)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    K33A I27C T174A T175N C287A

    Promoter

    unknown

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVTU_260_BirA

    Plasmid

    #63682

    Purpose

    Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

    Insert

    BirA
    

    Use

    Tags

    6X Histidine and TEV cleavage site

    Expression

    Yeast

    Mutation

    Promoter

    ADH1/yeast

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPL5606_TEF1*-Cre_ADE2

    Plasmid

    #60932

    Purpose

    Constitutive expression vector for Cre-recombinase containing ADE2 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. ykgMO-IGS-LuxCDABE

    Plasmid

    #207232

    Purpose

    Calprotectin sensing promoter with luciferase reporter

    Depositor

    Arthur Prindle

    Article

    Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    ykgMO-IGS
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Beta-Lactamase

    Plasmid

    #62729

    Purpose

    Expresses Beta-Lactamase protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    Beta-Lactamase
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Deletion of periplasmic signal domain

    Promoter

    T7

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PT7lacO-His6-AlaRS

    Plasmid

    #224362

    Purpose

    Express AlaRS protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    AlaRS (alaS E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPL5608_TEF1*-Cre_TRP1

    Plasmid

    #60931

    Purpose

    Constitutive expression vector for Cre-recombinase containing TRP1 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    Mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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