We narrowed to 9,122 results for: Pol

1. pSV(X) C/EBPalpha

   Plasmid

   #13392

   Depositor

   Richard Schwartz

   Article

   Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

   Insert

   C/EBPalpha (Cebpa Rat)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pCfB5191

   Plasmid

   #126911

   Purpose

   Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   X-4 and XII-5 gRNA
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMH-HT_CtCHAD

   Plasmid

   #127785

   Purpose

   Expresses CtCHAD in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain)

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3XFlag DVL2 (E499-G)

   Plasmid

   #24803

   Depositor

   Jeff Wrana

   Article

   Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

   Insert

   DVL2 (DVL2 Human)
   

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   E499G

   Available Since

   June 22, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pCellFree_G03 PDGFRB

   Plasmid

   #67130

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PDGFRB (PDGFRB Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pPRIME-TET-GIN-recipient

   Plasmid

   #11656

   Purpose

   3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP, IRES, and Neo

   Expression

   Mammalian

   Available Since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMH-HT_SsCHAD

   Plasmid

   #127783

   Purpose

   Expresses SsCHAD in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJin-290

   Plasmid

   #125718

   Purpose

   ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-C

   Depositor

   Bryan Dickinson

   Article

   Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

   Insert

   PCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
   

   Expression

   Mammalian

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac K-M33

   Plasmid

   #1986

   Depositor

   Robert Kingston

   Article

   Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

   Insert

   M33 (Cbx2 Mouse)
   

   Expression

   Insect

   Mutation

   Kozak sequence added before ATG

   Available Since

   March 23, 2006

   Availability

   Academic Institutions and Nonprofits only

10. psicheck-2-Tas2r3

    Plasmid

    #48176

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of TAS2R3 (TAS2R3 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pSCALPS_miR-146aSponge_mCherry_GFP

    Plasmid

    #149718

    Purpose

    Lentiviral vector expressing a miR-146a decoy sponge

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    14X miR-146a binding sites
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G to T mutation in position 75 of the insert

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330(puro) Rosa26-H3F3B

    Plasmid

    #73131

    Purpose

    Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

    Depositor

    Agnel Sfeir

    Article

    Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

    Insert

    spCas9
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX4T-1-human RNF146 RING-WWE (30-183)

    Plasmid

    #132611

    Purpose

    expresses human RNF146 RING-WWE (30-183) in E. coli

    Depositor

    Wenqing Xu

    Article

    DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

    Insert

    RNF146 RING-WWE (30–183)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

    Plasmid

    #172224

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMX156

    Plasmid

    #29482

    Depositor

    Linda Wordeman

    Article

    Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

    Insert

    Kif2A-beta (Kif2a Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD-DiB2

    Plasmid

    #168801

    Purpose

    Fluorogen activating protein (FAP) DiB2

    Depositor

    Alexander Mishin

    Article

    Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

    Insert

    Fluorogen activating protein DiB2
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Psy074

    Plasmid

    #99504

    Purpose

    Bacterial expression of LIPS M1+TE

    Depositor

    Jay Keasling

    Article

    Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

    Insert

    LIPS M1+TE
    

    Expression

    Bacterial

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. sm-9xGCN4-Renilla-MS2

    Plasmid

    #178009

    Purpose

    Expresses Spaghetti Monster (sm) 9xGCN4-Renilla mRNA reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Gomez-Puerta et al Elife. 2022 Jun 22;11. pii: 75580. doi:

    Insert

    sm-9xGCN4
    

    Expression

    Bacterial and Mammalian

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET:GACG:GFP

    Plasmid

    #60735

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLMB577

    Plasmid

    #83778

    Purpose

    Phenylalanine biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    promoter of RL1860
    

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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