We narrowed to 4,657 results for: lum

1. pQIq_MRGS_HIS8_(27F3)_FLAG

   Plasmid

   #199615

   Purpose

   Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-FLAG 27F3
   

   Tags

   8xHis and FLAG

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LRT_RTctrl

   Plasmid

   #214074

   Purpose

   Luminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. LRT

   Plasmid

   #214071

   Purpose

   Luminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

   Plasmid

   #175540

   Purpose

   Expression of HaloTag9 in the endoplasmic reticulum of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CalR-HaloTag9-KDEL
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_CalR_HaloTag7_KDEL

   Plasmid

   #175530

   Purpose

   Expression of HaloTag7 in the endoplasmic reticulum of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CalR-HaloTag7-KDEL
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. LiRT

   Plasmid

   #214075

   Purpose

   Luminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LiRT_RTctrl

   Plasmid

   #214078

   Purpose

   Luminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LiRTi

   Plasmid

   #214076

   Purpose

   Luminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LiRTX_ctrl

   Plasmid

   #214077

   Purpose

   Luminescent reporter system, NMD negative control. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Expression

   Mammalian

   Mutation

   Remnant of linker sequence present at the end of …

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT-Bip-GFP:V5:KDEL

    Plasmid

    #69917

    Purpose

    An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

    Depositor

    Kenneth Irvine

    Article

    Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

    Insert

    GFP
    

    Tags

    Drosophila BiP signal sequence, KDEL ER targeting…

    Expression

    Insect

    Promoter

    pMT

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAPV-DsRed2-ER-5

    Plasmid

    #170570

    Purpose

    Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-DsRed2-ER-5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMpGWB402-er-mCherry

    Plasmid

    #186288

    Purpose

    Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

    Insert

    er-mCherry
    

    Expression

    Bacterial

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCerulean3-CytERM-N-17

    Plasmid

    #55412

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    CytERM
    

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMDC32-Kar2-CFP-HDEL

    Plasmid

    #96989

    Purpose

    Express endoplasmic reticulum (ER) marker in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    Kar2-CFP-HDEL
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIgnaviCas9

    Plasmid

    #127595

    Purpose

    Expresses IgnaviCas9 in E. coli

    Depositor

    Stephen Quake

    Article

    Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

    Insert

    IgnaviCas9
    

    Tags

    6xHis Tag and Maltose binding protein

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRATIO3267

    Plasmid

    #141312

    Purpose

    Gateway cloning compatible dual bioluminescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    gLUC and redLUC

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRATIO1267

    Plasmid

    #141301

    Purpose

    Gateway cloning compatible dual bioluminescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    gLUC and redLUC

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQIq_MRGS_HIS8_(27G2)_FLAG

    Plasmid

    #199616

    Purpose

    Encodes DARPin-FLAG 27G2 for bacterial expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-FLAG 27G2
    

    Tags

    8xHis and FLAG

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-ER-rsTagRFP

    Plasmid

    #87620

    Purpose

    Endoplasmic reticulum (ER)-targeted rsTagRFP.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    ER-rsTagRFP
    

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177434

    Purpose

    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5r4 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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