We narrowed to 8,312 results for: Dos
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Plasmid#117530PurposeLevel0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)DepositorInsertSp-xCas9 3.7 (no stop codon)
UseCRISPRMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-xlH2B(1-114)_K114A
Plasmid#118217PurposeBacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.DepositorInsertxlH2B(1-114)
TagsMxe-GyrA intein fused to chitin binding domainExpressionBacterialMutationLysine 114 to alaninePromoterT7Available SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDA12
Plasmid#113663PurposeCloning vector for bacterial expression with Pdhfr promoterDepositorTypeEmpty backboneExpressionBacterialPromoterPdhfrAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPI184
Plasmid#113221Purposegeneric knockin vector for C-terminal tagging with mCherryDepositorTypeEmpty backboneUseGeneric knockin vector for c-terminal tagging wit…TagsmCherryAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPI192
Plasmid#113219Purposegeneric knockin vector for C-terminal tagging with mCherryDepositorTypeEmpty backboneUseGeneric knockin vector for c-terminal tagging wit…TagsmCherryAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_VCL_Vinculin
Plasmid#110045PurposeProtein expression and purification of VCL_VinculinDepositorInsertVCL_Vinculin (VCL Human)
ExpressionBacterialAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hook3(1-522)-GFP-Psc-StrepII
Plasmid#111861PurposeC-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.DepositorInsertHook3(1-552)-GFP (HOOK3 Human)
TagsGFP, Psc tag, and Strep tagExpressionInsectMutationOptimised for expression in SF9 cellsAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PfSSB_W166C
Plasmid#103001PurposeThe expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB (C93A,W166C), used for obtaining a fluorescent adduct at the cysteineDepositorInsertPfSSB_W166C
ExpressionBacterialMutationC93A, W166CPromoterT7Available SinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-T830E
Plasmid#91996PurposeExpresses FLAG-HA-AGO2 (T830E)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S834E
Plasmid#91998PurposeExpresses FLAG-HA-AGO2 (S834E)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FH-AGO2-S828A
Plasmid#91982PurposeExpresses FLAG-HA-AGO2 (S828A)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824A
Plasmid#91987PurposeExpresses FLAG-HA-AGO2 (S824A)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-T830A
Plasmid#91988PurposeExpresses FLAG-HA-AGO2 (T830A)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S831A
Plasmid#91989PurposeExpresses FLAG-HA-AGO2 (S831A)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S834A
Plasmid#91990PurposeExpresses FLAG-HA-AGO2 (S834A)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S828A
Plasmid#91991PurposeExpresses FLAG-HA-AGO2 (S828A)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824E
Plasmid#91994PurposeExpresses FLAG-HA-AGO2 (S824E)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S828E
Plasmid#91995PurposeExpresses FLAG-HA-AGO2 (S828E)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5A3-T
Plasmid#92019PurposeExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with N-terminal TEV cleavage site
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…MutationS219VAvailable SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom4.2
Plasmid#87347PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 4.2 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseExpressionBacterial and MammalianPromoter4.2 kbp proximal promoterAvailable SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only