We narrowed to 4,631 results for: lum

1. pAPV-DsRed2-mEmerald-ER-5

   Plasmid

   #170715

   Purpose

   Expresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   APV-DsRed2-mEmerald-ER-5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEE22

   Plasmid

   #161787

   Purpose

   Hi-SC2 reporter delivery plasmid

   Depositor

   François-Xavier Barre

   Article

   Espinosa et al Mol Cell. 2020 Sep 3;79(5):857-869.e3. d

   Insert

   Hi-SC2 reporter
   

   Use

   Conjugative plasmid to deliver hi-sc2 reporter

   Tags

   Expression

   Mutation

   Himar site included MmeI site, tandem loxP sites,…

   Promoter

   n/a

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. JGI_018307

   Plasmid

   #158831

   Purpose

   Rare earth element alcohol dehydrogenases

   Depositor

   Joint Genome Institute

   Article

   Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

   Insert

   Rhodovulum
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQP-2363

   Plasmid

   #138975

   Purpose

   Relocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illumination

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUPD-MTS2 (12)

   Plasmid

   #101698

   Purpose

   MTS2 in domestication vector

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Insert

   CCAT-[MTS from β subunit of Nicotiana plumbaginifolia F1 ATPase]-AATG
   

   Use

   Yeast expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mPapaya1-CytERM-N-17

   Plasmid

   #56352

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541

   Depositor

   Michael Davidson

   Article

   Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

   Insert

   CytERM
   

   Use

   Tags

   mPapaya1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMU-ERr

   Plasmid

   #61170

   Purpose

   Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   AtWAK2-mCherry-HDEL
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   April 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEos2-ER-5

   Plasmid

   #57373

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   ER (CALR Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pER-mScarlet3_N1

   Plasmid

   #189772

   Purpose

   Construct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   ER-mScarlet3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. ER-RpHLuorin2

    Plasmid

    #171717

    Purpose

    Luminal ER expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 targeted to ER
    

    Use

    Tags

    KDEL ER-retention signal and Signal sequence of h…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1+-Tornado-split-nLuc

    Plasmid

    #212612

    Purpose

    For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    Tornado-CVB3-split-nLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    C373T mutation in CVB3

    Promoter

    CMV

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. ZJOM90

    Plasmid

    #133647

    Purpose

    Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-SS-mCherry-HDEL
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_Floxed-STOP Fluc

    Plasmid

    #122962

    Purpose

    Firefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reaction

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_Floxed-STOP Fluc
    

    Use

    Cre/Lox and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ER-GCaMP3

    Plasmid

    #64854

    Purpose

    Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-GCaMP3
    

    Use

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH_Str-KDEL_neomycin

    Plasmid

    #65307

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    

    Use

    Lentiviral

    Tags

    KDEL motif and Signal Sequence

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDS221

    Plasmid

    #34981

    Purpose

    Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb1–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-Keima-cb5

    Plasmid

    #137755

    Purpose

    Targets Keima to the endoplasmic reticulum. Used to monitor ER-phagy

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    Keima-cb5
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEos2-Calreticulin-N-10

    Plasmid

    #57353

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Calreticulin (CALR Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. ZJOM144

    Plasmid

    #133630

    Purpose

    Integration of SS-GFP-HDEL in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae) Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-SS-GFP-HDEL
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDS220

    Plasmid

    #34980

    Purpose

    Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge