We narrowed to 15,071 results for: nol

1. pET28a-6xHis-MBP-TEV site-TadA8.20

   Plasmid

   #194702

   Purpose

   Expresses N-terminal MBP fused TadA8.20 in bacteria

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   TadA8.20
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   mutations in TadA are described in the paper

   Promoter

   T7 promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 ZoAl N-1F

   Plasmid

   #203763

   Purpose

   For expression of N-flag tagged ZoAl R2 protein in mammalian cells

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement protein
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG1171 FIREmate-ECFP-Giantin

   Plasmid

   #205166

   Purpose

   Expresses FIREmate-ECFP-Giantin in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   FIREmate-ECFP-Giantin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAMD1-2

   Plasmid

   #61564

   Purpose

   Standardized mini-Tn5 delivery plasmid, kanamycin resistant

   Depositor

   Víctor de Lorenzo

   Article

   Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

   Type

   Empty backbone

   Use

   Synthetic Biology; Mini-tn5 vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTlpA_mCherry

   Plasmid

   #229146

   Purpose

   Promoter strength evaluation of PtlpA

   Depositor

   Shrikrishnan Sankaran

   Article

   Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No

   Promoter

   PtlpA

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL2B -1538/+64

   Plasmid

   #24942

   Depositor

   Stephen Smale

   Article

   Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

   Insert

   IL-10 promoter (Il10 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB501NL3F10H

   Plasmid

   #141286

   Purpose

   No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Type

   Empty backbone

   Use

   Luciferase; Gateway

   Tags

   FLAG (x3), HIS (x10), and NLUC

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-TREX1

   Plasmid

   #27219

   Depositor

   Judy Lieberman

   Article

   Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   Jan. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-mE1

   Plasmid

   #32534

   Depositor

   Jorge Eduardo Azevedo

   Article

   Carvalho et al Mol Biotechnol. 2011 Oct 20.

   Insert

   E1, ubiquitin-activating enzyme (Uba1 Mouse)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2ST

    Plasmid

    #59384

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-dfrA
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDonor14-N7HELIX-KanR

    Plasmid

    #190663

    Purpose

    Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NA

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PCS2+Cas9-mSA

    Plasmid

    #103882

    Purpose

    Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    CAS9-MSA
    

    Use

    CRISPR

    Tags

    MSA (monomeric streptavidin

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. EasyFusion T2A-H2B-miRFP703

    Plasmid

    #113097

    Purpose

    General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-miRFP703
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAV-U6+27-Tornado-RhoBAST

    Plasmid

    #196341

    Purpose

    Overexpression of circular RhoBAST

    Depositor

    Murat Sunbul

    Article

    Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

    Insert

    Tornado-RhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD-HisD-miniGFP2

    Plasmid

    #188897

    Purpose

    Express 6xHis-Tag miniGFP2 in E.coli

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. EasyFusion T2A-H2B-mCherry

    Plasmid

    #113089

    Purpose

    General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV_Cas9_puro

    Plasmid

    #65655

    Purpose

    Retroviral introduction of Cas9 into mammalian cell line.

    Depositor

    Christopher Vakoc

    Article

    Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    5 ' FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    MSSV_LTR

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG1237 FIREmate-ECFP-Fis1

    Plasmid

    #205173

    Purpose

    Expresses FIREmate-ECFP-Fis1 in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    FIREmate-ECFP-Fis1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge