We narrowed to 8,492 results for: sars
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Plasmid#131114PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C355A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 355 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C344A-2xStrep
Plasmid#131115PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C344A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 344 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C341A-2xStrep
Plasmid#131116PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C341A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 341 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C301A-2xStrep
Plasmid#131117PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C301A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 301 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C298A-2xStrep
Plasmid#131118PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C298A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 298 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C295A-2xStrep
Plasmid#131119PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C295A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 295 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C289A-2xStrep
Plasmid#131120PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C289A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 289 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C244A-2xStrep
Plasmid#131121PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C244A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 244 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 200-429-2xStrep
Plasmid#130955PurposeExpresses KSHV ORF66 a.a. 200-429 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 200-429PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C427A-2xStrep
Plasmid#131109PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C427A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 427 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C424A-2xStrep
Plasmid#131110PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C424A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 424 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)
Plasmid#182287PurposeEncodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
Tagsnuclear export signal (NES)ExpressionMammalianMutationY306A/Y384F (AF) double mutant of Methanosarcina …PromoterCMV and U6Available SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRaGE Pyl TAG GFP Y35TAG
Plasmid#160041PurposeEncodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.DepositorInsertsPyrrolysyl tRNA synthetase (Methanosarcina mazei)
Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
deGFP
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationChanged tyrosine 35 to TAG stop-codon (Site numbe…PromoterP70b promoter and T500 terminator, Vibrio natrieg…Available SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)
Plasmid#182652PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
4xU6-tRNAM15
Tagsnuclear export signal (NES)ExpressionMammalianMutationY306A/Y384F (AF) double mutant of Methanosarcina …PromoterCMV and U6Available SinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en31FnCas9ABEmax8.17d
Plasmid#201956PurposeMammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNADepositorInsertbpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianPromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-dFnCas9
Plasmid#201954PurposeMammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-dFnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationD11A and H969A on FnCas9PromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-en1dFnCas9GFP
Plasmid#201950PurposeExpression of en1dFnCas9-GFP in bacterial cellsDepositorInsertExpression of en1dFnCas9-GFP in bacterial cells
UseCRISPRTags6x His, HA, NLS, and mEGFPExpressionBacterialMutationE1369R on FnCas9PromoterT7Available SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-en15dFnCas9GFP
Plasmid#201951PurposeExpression of en15dFnCas9-GFP in bacterial cellsDepositorInsertExpression of en15dFnCas9-GFP in bacterial cells
UseCRISPRTags6x His, HA, NLS, and mEGFPExpressionBacterialMutationE1603H on FnCas9PromoterT7Available SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-dSpCas9
Plasmid#201953PurposeMammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-dSpCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A on SpCas9PromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA
Plasmid#182653PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
mutant eukaryotic release factor 1
TagsHA tag and nuclear export signal (NES)ExpressionMammalianMutationE55D and Y306A/Y384F (AF) double mutant of Methan…PromoterCMV and U6Available SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only