We narrowed to 9,122 results for: Pol

1. CAG-EGFP-Otx2shRNA1

   Plasmid

   #73981

   Purpose

   Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse Otx2 gene
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRISPRmTmG2

   Plasmid

   #69992

   Purpose

   The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   gRNA that targets near LoxP sites
   

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM149

   Plasmid

   #134178

   Purpose

   Bacterial expression of his-CENP-C 510-end

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

   Insert

   his-CENP-C 510-end (CENPC Human)
   

   Expression

   Bacterial

   Mutation

   aa 510-end

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCellFree_G03 PPARG

   Plasmid

   #67099

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PPARG (PPARG Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pLMB687

   Plasmid

   #83781

   Purpose

   Sucrose biosensor plasmid

   Depositor

   Philip Poole

   Article

   Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

   Insert

   Region upstream pRL120556 containing all of pRL120555
   

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSicoR-mCh-Chd1i4

   Plasmid

   #21908

   Depositor

   Miguel Ramalho-Santos

   Article

   Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

   Insert

   Chd1-i4
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBM23-pyrF-NT

   Plasmid

   #174384

   Purpose

   pBM23 contained pyrF and mini-CRISPR.

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   pyrF-NT
   

   Use

   Template for cloning fragments for chromosomal in…

   Expression

   Bacterial

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHFX-B60-EPSUD

   Plasmid

   #174390

   Purpose

   Plasmids for gene knockout were constructed based on suicide plasmid pHFX-B60

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   EPSUD
   

   Use

   Archaeal gene knockout vector for haloferax medit…

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBM23-pyrF-CS

   Plasmid

   #174396

   Purpose

   pBM23 contained pyrF and mini-CRISPR.

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   pyrF-CS
   

   Use

   Template for cloning fragments for chromosomal in…

   Expression

   Bacterial

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CTAG_linker-eGFP

    Plasmid

    #136098

    Purpose

    FP for C-term fusion with CDS12 without linker

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_AlaSerGly10xAlaGlySer_eGFP
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CTAG_linker-mTurquoise2

    Plasmid

    #136100

    Purpose

    FP for C-term fusion with CDS12 without linker

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_AlaSerGly10xAlaGlySer_mTurquoise2
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. CDS_GUS

    Plasmid

    #136084

    Purpose

    For histochemical _-glucuronidase activity assays. This GUS CDS does not have an intron.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS_GUS
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. CDS12_eGFP-linker

    Plasmid

    #136086

    Purpose

    FP for N-term fusion with a CTAG without linker

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS12_eGFP(noStop)-SerGly10xAlaSer
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CDS12_mScarletI-linker

    Plasmid

    #136088

    Purpose

    FP for N-term fusion with a CTAG without linker

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS12_mScarletI(noStop)-SerGly10xAlaSer
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pMK110

    Plasmid

    #99535

    Purpose

    contains bgl operon sequence for E. coli in vitro transcription template

    Depositor

    Robert Landick

    Article

    Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife

    Insert

    E. coli bgl antisense region containing bglG
    

    Tags

    None

    Promoter

    lambda Pr promoter used for in vitro transcriptio…

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBW-m4046802

    Plasmid

    #145683

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCellFree_G03 PSMC1

    Plasmid

    #67087

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PSMC1 (PSMC1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Mutation

    E275G, I287V and R294G

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pGEX-5X-1-DJ1-D149A

    Plasmid

    #29351

    Depositor

    Mark Cookson

    Article

    Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

    Insert

    DJ1 (PARK7 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Asp149Ala

    Available Since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

19. FT1

    Plasmid

    #31435

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Fused Toes Homolog (AKTIP Human)
    

    Tags

    HA

    Expression

    Yeast

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

20. Syn3D33A

    Plasmid

    #13400

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector. D33 of s…

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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