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TREt-Kras(G12D)-PGK-rtTA3

Plasmid

#256624

Purpose

An "All-in-One" Sleeping Beauty (SB) transposon vector for doxycycline-inducible expression of oncogenic Kras(G12D).

Depositor

Wen Xue

Article

Qiao et al Adv Sci (Weinh). 2026 Jan;13(6):e11312.

Inserts

Kras (Kras Mouse)
rTertR

Expression

Mammalian

Mutation

G12D

Promoter

Ef1a and PGK

Available Since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_N22_HA_hsAdar2

Plasmid

#250536

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of N22 peptide fused to the catalytic domain of human Adar2

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

N22_HA_hsAdar2 (ADARB1 Synthetic)

Use

Synthetic Biology

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_2xRecEnhSTOP_EcoRI_EYFP

Plasmid

#250547

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing an EcoRI site with two editible stop codons

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_2xRecEnhSTOP_EcoRI_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_1xRecEnhSTOP_EcoRI_EYFP

Plasmid

#250548

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing an EcoRI site with one editible stop codon

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_1xRecEnhSTOP_EcoRI_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_hsPum2_hsAdar2

Plasmid

#250539

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of human Pum2 fused to the catalytic domain of human Adar2

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

hsPum2_hsAdar2 (ADARB1, PUM1 Human)

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_hsAdar2_E488Q_T375G

Plasmid

#250537

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of human Adar2 (E488Q, T375G)

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

hsAdar2_E488Q_T375G (ADARB1 Human)

Use

Synthetic Biology

Mutation

E488Q, T357G

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_drePum1_HA_dreAdar1

Plasmid

#250541

Purpose

In vitro transcription (SP6 promoter) or mammalian expression (CMV promoter) of zebrafish Pum1 fused to the catalytic domain of zebrafish Adar1

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

drePum1_HA_dreAdar1

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_hsPum2_dreAdar1

Plasmid

#250542

Purpose

In vitro transcription (SP6 promoter) or mammalian expression (CMV promoter) of human Pum2 fused to the catalytic domain of zebrafish Adar1

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

hsPum2_dreAdar1 (PUM2 Synthetic)

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_EYFP_3xBoxB_Recorder_SV40

Plasmid

#250534

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a recorder containing 3 box-b hairpins

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

EYFP_3xBoxB_Recorder_SV40

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_N22_HA_dreAdar1

Plasmid

#250532

Purpose

In vitro transcription (SP6 promoter) or mammalian expression (CMV promoter) of N22 peptide fused to the catalytic domain on zebrafish Adar1

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

N22_HA_dreAdar1

Use

Synthetic Biology

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-mTDGa.1-BioID2-HA

Plasmid

#232028

Purpose

mammalian expression (CMV promoter) of murine TDGa.1 (catalytic mutant N151A) fused to BioID2-HA

Depositor

Primo Schaer

Article

Richina et al Cell Mol Life Sci. 2025 Nov 25. doi: 10.

Insert

Tdg (Tdg Mouse)

Tags

BioID2-HA

Expression

Mammalian

Mutation

N151A (site-directed mutagenesis: aac -> gcc)

Promoter

CMV

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mTDGa.2-BioID2-HA

Plasmid

#232029

Purpose

mammalian expression (CMV promoter) of TDGa.2 (SIM mutant E321Q) fused to BioID2-HA

Depositor

Primo Schaer

Article

Richina et al Cell Mol Life Sci. 2025 Nov 25. doi: 10.

Insert

Tdg (Tdg Mouse)

Tags

BioID2-HA

Expression

Mammalian

Mutation

K341A (site directed mutagenesis: aag -> aag)

Promoter

CMV

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-TRIP12(749-836)-EGFP

Plasmid

#196229

Purpose

EGFP fused to the C-terminus of a WWE domain within TRIP12 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged TRIP12(749- 836)

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-DTX2(8-97)-EGFP

Plasmid

#196230

Purpose

EGFP fused to the C-terminus of first WWE domain within Deltex2 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged DTX2(8-97)

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-DTX2(8-174)-EGFP

Plasmid

#196232

Purpose

EGFP fused to the C-terminus of tandem WWE domains within Deltex2 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX2(8-174)-EGFP

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(WT)-EGFP

Plasmid

#196233

Purpose

EGFP fused to the C-terminus of Af1521 (WT) macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged Af1521(WT)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP

Plasmid

#196235

Purpose

EGFP fused to the C-terminus of Af1521(K35E/Y145R) macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged Af1521(K35E/Y145R)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

K35E and Y145R

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-RNF146(100-182)-myc

Plasmid

#196236

Purpose

RNF146(100-182) WWE domain with a myc tag fused to the C terminus & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146(100-182) encoding a C-terminal myc tag

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(K35E/Y145R)-myc

Plasmid

#196237

Purpose

Af1521(K35E/Y145R) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

Af1521(K35E/Y145R) encoding a C-terminal myc tag

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Mutation

amino acid 35 lysine is replaced with glutamic ac…

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(G42E)-myc

Plasmid

#196238

Purpose

Af1521(G42E) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

Af1521(G42E)-myc

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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