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pLenti-CMV-Puro-PNPT1 S484A

Plasmid

#223316

Purpose

Lentiviral vector expressing human PNPT1 S484A variant

Depositor

Judy Lieberman

Publication

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed serine 484 to alanine

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

Plasmid

#224497

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax

Use

Gateway Cloning

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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RPL13-ECFP-SNAP

Plasmid

#187967

Purpose

expresses RPL13 with C-terminal tags ECFP and SNAP

Depositor

Markus Wahl

Publication

Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

Insert

RPL13 (RPL13 Human)

Tags

ECFP-SNAP

Expression

Mammalian

Promoter

CMV

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA GFP10-RHOA

Plasmid

#133068

Purpose

Expresses RHOA GTPase with tripartite split GFP10

Depositor

Stéphanie Cabantous

Publication

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

ras homolog family member A (RHOA Human)

Tags

GFP10 (split-GFP tripartite)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-fCD200

Plasmid

#164425

Purpose

AAV plasmid expressing full-length CD200 in photoreceptors

Depositor

Connie Cepko

Publication

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

CD200 (Cd200 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3/flag-pBirA(1-G78)-FRB

Plasmid

#154447

Purpose

expresses pBirA(1-G78)-FRB/biochemical assay

Depositor

Hyun-Woo Rhee

Publication

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

flag-pBirA(1-G78)-FRB

Expression

Mammalian

Promoter

T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 deltaNCD

Plasmid

#124920

Purpose

For expression of N-terminal Conserved Domain deletion mutant of SIN1-GFP (delta2-137)

Depositor

Jin Chen

Publication

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2 Halo-Tev- HSPB7 (zebrafish) WT

Plasmid

#108916

Purpose

Express WT Zebrafish Halo-Tev-HSPB7 in mammalian cells

Depositor

Yimon Aye

Publication

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

Halo-TEV-HSPB7 (hspb7 Zebrafish)

Tags

Halo-TEV

Expression

Mammalian

Promoter

SP6

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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cmamxA1-GFP

Plasmid

#107195

Purpose

Expresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

Depositor

Volker Gerke, Ursula Rescher

Publication

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

cmannexin A1

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

exchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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mOC-STAMP_3xFlag-Myc-CMV26

Plasmid

#73577

Purpose

Express mouse OC-STAMP in mammalian cells

Depositor

Paul Odgren

Publication

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Insert

OC-STAMP (Ocstamp Mouse)

Tags

3x Flag and c-Myc tag

Expression

Mammalian

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
p3xFLAG-Tmprss6(Mask)

Plasmid

#18790

Depositor

Bruce Beutler

Publication

Du et al Science. 2008 May 23. 320(5879):1088-92.

Insert

Type II transmembrane serine protease 6 (Tmprss6 Mouse)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 567-811 and replaced with 28 …

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE

Plasmid

#236239

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Publication

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV2.1 (CACNA1A Rabbit)

Use

Lentiviral

Tags

mEmerald

Promoter

human Synapsin 1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

Plasmid

#236246

Purpose

Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

Depositor

Jennifer Lippincott-Schwartz

Publication

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

U6 and human Synapsin1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mNG-eDHFR(69K6)-G-alpha-q

Plasmid

#172106

Purpose

Mammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Publication

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Gαq (GNAQ Human)

Expression

Mammalian

Mutation

Gαq: C9S/C10S/Q209L

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR-Kras

Plasmid

#123357

Purpose

FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

Depositor

Jin Zhang

Publication

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR-Kras

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pET20b_Pa-PiuA

Plasmid

#128955

Purpose

expresses Pseudomonas aeruginosa PiuA in E. coli

Depositor

James Naismith

Publication

Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

Insert

iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)

Tags

6xHis and pelB leader

Expression

Bacterial

Mutation

Signal peptide sequence has been removed

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1-HLx10

Plasmid

#108137

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Publication

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]10:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]10:SACM1L(521-587)

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pVENUS-N1-Ubl4A

Plasmid

#86857

Purpose

To express Ubl4A tagged with VENUS at C terminus.

Depositor

Yihong Ye

Publication

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

VENUS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-SLC30A10-E25A

Plasmid

#82345

Purpose

Expresses FLAG-tagged SLC30A10-E25A in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Publication

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

E25A

Promoter

CMV

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

Plasmid

#80272

Purpose

mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

Depositor

Andrew Holland

Publication

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of multi-phospho degron (amino acids 282…

Promoter

CMV

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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