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pLAP-BUBR1-K795A

Plasmid

#114061

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

Insert

BUBR1-K795A (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-K795A

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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CDC7 gRNA (BRDN0001146695)

Plasmid

#76683

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDC7 (CDC7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CDC7 gRNA (BRDN0001149152)

Plasmid

#76684

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDC7 (CDC7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CDC7 gRNA (BRDN0001149135)

Plasmid

#76685

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDC7 (CDC7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP3K5 gRNA (BRDN0001147010)

Plasmid

#76653

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K5 (MAP3K5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP3K5 gRNA (BRDN0001148021)

Plasmid

#76655

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K5 (MAP3K5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP3K5 gRNA (BRDN0001147988)

Plasmid

#76656

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K5 (MAP3K5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
BUB1B gRNA (BRDN0001148077)

Plasmid

#75918

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BUB1B (BUB1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
BUB1B gRNA (BRDN0001146079)

Plasmid

#75919

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BUB1B (BUB1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
BUB1B gRNA (BRDN0001147884)

Plasmid

#75920

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BUB1B (BUB1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge