We narrowed to 4,251 results for: Gca

1. pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1

   Plasmid

   #158758

   Purpose

   Cell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).

   Depositor

   Edward Boyden

   Article

   Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

   Insert

   nullCoChR-GCaMP6f-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9

   Plasmid

   #87917

   Purpose

   Gateway cloning

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   sgHTT1, sgGFP2, shCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1, U6, 7SK

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-GFAP-jGCaMP7c variant 1513

   Plasmid

   #173159

   Purpose

   Expresses jGCaMP7c variant 1513 in astrocytes

   Depositor

   Hervé Chneiweiss, Vincent Vialou

   Insert

   jGCaMP7c variant 1513-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   GFAP

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV1-EF1α-F-Flex-jGCaMP7b-WPR

   Plasmid

   #171689

   Purpose

   Flp-dependent expression of jGCaMP7b

   Depositor

   Mark Huebener, Tobias Bonhoeffer, Tobias Rose

   Article

   Bauer et al Neuron. 2021 Aug 4;109(15):2457-2468.e12

   Insert

   GCaMP7b
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck

   Plasmid

   #58522

   Purpose

   Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   sdChR(C138S)-TS-GCaMP6f-ER
   

   Use

   Lentiviral

   Tags

   GCaMP6f

   Expression

   Mammalian

   Mutation

   changed Cysteine 138 to Serine

   Promoter

   a-CamKII

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX552-hSyn-DIO-GCaMP8f_Grin1 gRNA

   Plasmid

   #215281

   Purpose

   Expresses Grin 1 gRNA in a Cre dependent GCaMP8f vector

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   Grin1 gRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   U6

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX552-hSyn-DIO-GCaMP8f_Grin1TTT gRNA

   Plasmid

   #215282

   Purpose

   Expresses Grin1 Control gRNA in a Cre dependent GCaMP8f vector

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   Grin1 control gRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO-GCaMP6f-WPRE

   Plasmid

   #216275

   Purpose

   AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6f

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   GCaMP6f
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Expression

   Mutation

   Promoter

   human Synapsin 1 (hSyn)

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-FAS-GCaMP6f-WPRE

   Plasmid

   #141237

   Purpose

   AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   GCaMP6f
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Expression

   Mutation

   Promoter

   Human elongation factor-1 alpha (EF-1 alpha)

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-ef1a-DIO-eGCaMP(plus)

    Plasmid

    #201149

    Purpose

    AAV virus production for Cre recombinase dependent expression of eGCaMP(plus)

    Depositor

    Andre Berndt

    Article

    Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

    Insert

    eGCaMP+
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFR-mScarletI-antiFRET-P2A-GCaMP3

    Plasmid

    #191460

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) GCaMP3 to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    GCaMP3
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFR-mScarletI-antiFRET-P2A-jGCaMP7c

    Plasmid

    #191461

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) jGCaMP7c to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    jGCaMP7c
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-syn-LifeAct-jGCaMP8f-WPRE

    Plasmid

    #186038

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8f under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8f
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-syn-LifeAct-jGCaMP8s-WPRE

    Plasmid

    #186040

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8s under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8s
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CaMKII-GCaMP6f-4x128-mAGNET

    Plasmid

    #166023

    Purpose

    Expresses GCaMP6f in CaMKII+ cells with low miRNA-128 expression

    Depositor

    Xue Han

    Article

    Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

    Insert

    GCaMP6f with 4 miR-128 binding sites
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

    Plasmid

    #104495

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, and AAV1

    Insert

    jGCaMP7s
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51087

    Purpose

    Forebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa 1.3 kb

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3

    Plasmid

    #112005

    Purpose

    For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV5 and AAV9

    Insert

    axon-GCaMP6s-P2A-mRuby3
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV Cag Flex H2B Gcamp6f reverse

    Plasmid

    #74155

    Purpose

    Calcium Sensor

    Depositor

    Loren Looger

    Insert

    Gcamp6f
    

    Use

    AAV

    Tags

    H2B

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)

    Plasmid

    #163047

    Purpose

    GCaMPer calcium sensor (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMPer
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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