We narrowed to 4,010 results for: SPL

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pETcon-pGAL1-Aga2-Amyloid β42-c_myc

Plasmid

#225223

Purpose

Yeast optimized human Amyloid β42 fused with aga2 protein gene under gal1 promoter for the expression of Amyloid β42 in yeast surface display.

Depositor

Urartu Seker

Article

Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

Inserts

Amyloid β42
Aga2 (AGA2 Budding Yeast)

Tags

HA Tag and c-myc Tag

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pFlare9A-Clip2E9 Mutant

Plasmid

#209575

Purpose

minigene construct for Clip2 E9 alternative splicing, potential PTBP2 binding sites are deleted

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Clip2 (Clip2 Mouse)

Expression

Mammalian

Mutation

ACGCGTTTCTGAATTCTTCTAACTGCCCTCAAATGCACGGTGGCATGTG…

Promoter

CMV

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-THRA-RFP-EGFP

Plasmid

#228537

Purpose

Bichromatic fluorescent reporter of THRA isoform 1 and isoform 2 splicing in mammalian cells

Depositor

Markus Schuelke

Article

Graceffo et al Int J Mol Sci. 2024 Dec 17;25(24):13512.

Insert

Thyroid Hormone Receptor Alpha (THRA Human)

Tags

eGFP and tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCR2.1_HBB_A

Plasmid

#87848

Purpose

pCR2.1-based plasmid holding a 328-bp fragment containing the exon-1-exon-2 splice border of aberrant HBB[IVSI-110(G>A)] human β-globin mRNA

Depositor

Marina Kleanthous

Article

Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

Insert

Homo sapiens hemoglobin subunit beta (HBB), Aberrant cDNA fragment (Exon1/19nt mispliced Intron1 + Exon2) (HBB Human)

Expression

Bacterial

Mutation

HBB(IVSI-110) β-thalassaemia misplicing mutation …

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJF3H

Plasmid

#63897

Purpose

For phage display of cDNA libraries

Depositor

Carlos Barbas

Article

Barbas et al Cold Spring Harbor, NY: Cold Spring Harb

Insert

cDNA cloning region

Use

Phage display

Tags

Fos

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pB33eCPX

Plasmid

#23336

Depositor

Patrick Daugherty

Article

Rice et al Protein Eng Des Sel. 2008 Jul . 21(7):43

Insert

Enhanced Circularly Permuted Outer Membrane Protein X

Expression

Bacterial

Available Since

March 9, 2010

Availability

Academic Institutions and Nonprofits only

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p2-39

Plasmid

#118086

Purpose

ZB-linker-split C-terminal T7 RNAP expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

ZB-linker-split C-terminal T7 RNAP expression plasmid

Use

Synthetic Biology

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p5-71

Plasmid

#118085

Purpose

N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid

Use

Synthetic Biology

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p5-39

Plasmid

#118088

Purpose

FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid

Use

Synthetic Biology

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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HC-CfaN

Plasmid

#122951

Purpose

Mammalian codon optimized N-intein

Depositor

Thomas Muir

Article

Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

Insert

CfaN

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET_mNG2(1-10)_32aalinker_mNG2(11)

Plasmid

#82611

Purpose

Bacterial expression vector for split mNeonGreen2 screening

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)

Expression

Bacterial

Mutation

split before mutations

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTXB1-CfaC-Gyra

Plasmid

#122950

Purpose

Bacterial expression of C-intein

Depositor

Thomas Muir

Article

Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

Insert

CfaC

Expression

Bacterial

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSKDuet01

Plasmid

#12172

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

NpuDnaE

Tags

His-GB1

Expression

Bacterial

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pSKBAD2

Plasmid

#15335

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein

Tags

GB1

Expression

Bacterial

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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p5-79

Plasmid

#118087

Purpose

N-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

N-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid

Use

Synthetic Biology

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSZBAD43

Plasmid

#15336

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CYN)

Tags

GB1

Expression

Bacterial

Mutation

F to Y mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pJJBAD80

Plasmid

#15346

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CAN)

Tags

GB1

Expression

Bacterial

Mutation

F to A mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pSZBAD44

Plasmid

#15337

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CTN)

Tags

GB1

Expression

Bacterial

Mutation

F to T mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pSZBAD45

Plasmid

#15338

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CRN)

Tags

GB1

Expression

Bacterial

Mutation

F to R mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pSZBAD47

Plasmid

#15339

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CWN)

Tags

GB1

Expression

Bacterial

Mutation

F to W mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only