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pAAV TRE3G:RiboL1-jGCaMP8f

Plasmid

#239677

Purpose

Soma-targeted (ribosome tethered via RiboL1) jGCaMP8f expressed under a TRE3G (tet responsive element) promoter.

Depositor

Marianne Fyhn

Article

Otero-Coronel et al bioRxiv 2026.05.28.728595

Insert

jGCaMP8f

Use

AAV

Tags

6xHis and RiboL1

Expression

Mammalian

Promoter

TRE3G

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV TRE3G:RiboL1-jGCaMP8m

Plasmid

#239678

Purpose

Soma-targeted (ribosome tethered via RiboL1) jGCaMP8m expressed under a TRE3G (tet responsive element) promoter.

Depositor

Marianne Fyhn

Article

Otero-Coronel et al bioRxiv 2026.05.28.728595

Insert

jGCaMP8m

Use

AAV

Tags

6xHis and RiboL1

Expression

Mammalian

Promoter

TRE3G

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV TRE3G:Soma-jGCaMP8s

Plasmid

#239679

Purpose

Soma-jGCaMP8s expressed under a TRE3G (tet responsive element) promoter.

Depositor

Marianne Fyhn

Article

Otero-Coronel et al bioRxiv 2026.05.28.728595

Insert

Soma-jGCaMP8s

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

TRE3G

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-Gfa104-jGCaMP8f

Plasmid

#254431

Purpose

For production of AAV containing jGCaMP8f

Depositor

Christopher Norris

Article

Weiss et al J Neurosci. 2025 Oct 1;45(40):e034925202

Insert

fast calcium green fluorescent indicator

Use

AAV

Promoter

Gfa104

Available Since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-Gfa104-jGCaMP8m

Plasmid

#254432

Purpose

For production of AAV containing jGCaMP8m

Depositor

Christopher Norris

Insert

medium fast calcium green fluorescent indicator

Use

AAV

Promoter

Gfa104

Available Since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG2-GCaMP6s

Plasmid

#241022

Purpose

An AAV transfer plasmid expressing the GCaMP6s genetically encoded calcium indicator under control of the ubiquitously expressed CAG2 promoter.

Depositor

Thomas Johnson

Article

Aguzzi et al Sci Transl Med. 2026 Apr 29;18(847):eadr

Insert

GCaMP6s

Use

AAV

Tags

6X His tag

Promoter

CAG2

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1-CAG-GCaMP6s

Plasmid

#241023

Purpose

An HDR plasmid for the AAVS1 safe harbor locus carrying a gene cassette consisting of GCaMP6s under control of a CAG promoter and a WPRE.

Depositor

Thomas Johnson

Article

Aguzzi et al Sci Transl Med. 2026 Apr 29;18(847):eadr

Insert

GCaMP6s and WPRE

Use

CRISPR

Tags

6x His Tag

Promoter

CAG

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS4-GCaMP6f

Plasmid

#209931

Purpose

Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter. Based on construct from Pouchelon et al Cell Rep Methods. 2022.

Depositor

Jessica Cardin

Insert

GCaMP6f

Use

AAV, CRISPR, and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET30b jGCaMP8f L27A

Plasmid

#204138

Purpose

Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Bacterial

Mutation

Leucine 27 changed to Alanine

Promoter

T7

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD4-GCaMP6f

Plasmid

#178727

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6-GCaMP6f

Plasmid

#178729

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-GCaMP6f

Plasmid

#178731

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS6-GCaMP6f

Plasmid

#178732

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAS1-his-24::GCaMP6s

Plasmid

#124333

Purpose

Expressing Nuclear Localized GCaMP6s

Depositor

Andrew Leifer

Insert

GCaMP6s (his-24 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-GCaMP6f-WPRE

Plasmid

#141237

Purpose

AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

GCaMP6f

Use

AAV and Cre/Lox

Tags

6XHIS and Xpress

Promoter

Human elongation factor-1 alpha (EF-1 alpha)

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Adamts2.1-DIO-GCaMP8s-WPRE

Plasmid

#211491

Purpose

conditional expression of GCaMP8s under artificial promoter Adamts2

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

DIO-GCaMP8s

Use

AAV

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Baz1a.1-DIO-GCaMP8s-WPRE

Plasmid

#211493

Purpose

conditional expression of GCaMP8s under artificial promoter Baz1a

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

DIO-GCaMP8s

Use

AAV

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV1-EF1α-F-Flex-jGCaMP7b-WPR

Plasmid

#171689

Purpose

Flp-dependent expression of jGCaMP7b

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Bauer et al Neuron. 2021 Aug 4;109(15):2457-2468.e12

Insert

GCaMP7b

Use

AAV

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-jGCaMP7c variant 1513

Plasmid

#173159

Purpose

Expresses jGCaMP7c variant 1513 in astrocytes

Depositor

Hervé Chneiweiss, Vincent Vialou

Insert

jGCaMP7c variant 1513-WPRE

Use

AAV

Promoter

GFAP

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

Plasmid

#199580

Purpose

Expresses GCaMP8f in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

His-DIO GCaMP8f

Use

AAV, CRISPR, and Cre/Lox

Tags

6xHis

Expression

Mammalian

Promoter

hsyn

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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