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pcDNA3.1-STIM1-Venus-173-C

Plasmid

#87619

Purpose

Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

STIM1-Venus173-C (STIM1 Human, Synthetic)

Expression

Mammalian

Promoter

CMV

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-Venus-173-N-ORAI1

Plasmid

#87618

Purpose

N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Venus173-N-ORAI1 (ORAI1 Human, Synthetic)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

Expression

Mammalian

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJEC779

Plasmid

#234348

Purpose

Modular RENDR, GFP output, targets synthetic target

Depositor

James Chappell

Publication

Gambill et al Nat Commun. 2023 Feb 1;14(1):543. doi: 1

Insert

Modular RENDR, GFP output

Use

Synthetic Biology

Promoter

PJ23119

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pJEC780

Plasmid

#234349

Purpose

Expressed synthetic RNA target for RENDR

Depositor

James Chappell

Publication

Gambill et al Nat Commun. 2023 Feb 1;14(1):543. doi: 1

Insert

Synthetic RNA target for RENDR

Use

Synthetic Biology

Promoter

PJ23119

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-1 Sesn1b

Plasmid

#61874

Purpose

bacterial expression of a GST-Sesn1b (mouse)

Depositor

Ming Li

Publication

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn1 (Sesn1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

none

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-6p-1 Sesn2

Plasmid

#61873

Purpose

bacterial expression of a GST-Sesn2 (mouse)

Depositor

Ming Li

Publication

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Tags

GST

Expression

Bacterial

Mutation

none

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)

Plasmid

#39859

Depositor

Jonathon Pines

Publication

Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

Insert

C11orf51 (ANAPC15 Human)

Tags

3xFlag

Expression

Mammalian

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
pENTR SD Age HsSAS-6

Plasmid

#46381

Purpose

For expression of C-terminal fusions.

Depositor

Pierre Gonczy

Publication

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Gateway Cloning

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-ExRai-AktAR(T/A)

Plasmid

#118463

Purpose

Negative-control mutant for ExRai-AktAR biosensor.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AktAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HER3-DMVF-181-R

Plasmid

#87621

Purpose

C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

HER3-DMVF181-C (ERBB3 Human, Synthetic)

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag-Sesn2

Plasmid

#61871

Purpose

Lentivirial expression of Flag-Sesn2 (mouse)

Depositor

Ming Li

Publication

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
pInducer21 Flag-Sesn2

Plasmid

#61829

Purpose

Dox inducible lentivirial expression of Flag-Sesn2 (mouse)

Depositor

Ming Li

Publication

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Flag-Sesn1b

Plasmid

#61867

Purpose

Transient overexpression of Flag-Sesn1b (mouse)

Depositor

Ming Li

Publication

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn1 (Sesn1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-RAB-EKARev(T/A)

Plasmid

#118475

Purpose

Negative-control mutant for RAB-EKARev biosensor.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKI-GFP

Plasmid

#118482

Purpose

GFP-tagged Protein Kinase A inhibitor peptide.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

PKI-GFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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single polypeptide FPX biosensor for both caspase-3 and -8

Plasmid

#60886

Purpose

FPX biosensor

Depositor

Robert Campbell

Publication

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

single polypeptide FPX biosensor for caspase-3 and 8

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3-PhoDer

Plasmid

#188473

Purpose

Expresses the optogenetic human Gasdermin D (PhoDer) in mammalian cells

Depositor

Gary Mo

Publication

Santa Cruz Garcia et al Nat Commun. 2022 Jan 10;13(1):52. doi: 1

Insert

PhoDer (GSDMD Human, Synthetic)

Expression

Mammalian

Promoter

CMV IE

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-3xflagGREB1a

Plasmid

#112221

Purpose

mammalian expression of flag tagged GREB1a

Depositor

Craig Burd

Publication

Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

Insert

GREB1a (GREB1 Human)

Tags

3x flag

Expression

Mammalian

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H2B-Venus

Plasmid

#87614

Purpose

Venus (YFP) fused to histone H2B.

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

H2B-Venus (H2BC8 Human, Synthetic)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR Age HsSAS-6 ST

Plasmid

#46382

Purpose

For expression of N-terminal fusions.

Depositor

Pierre Gonczy

Publication

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Gateway Cloning

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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