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pLKO.1-blast-cfRab25_2

Plasmid

#26711

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to canine Rab25.

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only

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3280 pcDNA3 Bid p22 cDNA

Plasmid

#8767

Depositor

Stanley Korsmeyer

Article

Wang et al Genes Dev 1996 Nov 15;10(22):2859-69.

Insert

Bid p22 ORF cDNA FL (Bid Mouse)

Expression

Mammalian

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pM2_J23102_R1_LuxR

Plasmid

#250887

Purpose

LuxR regulator protein expressed under BBa_J23102 promoter; part of BNeu AS4, AS8, AS9 and AS14; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

LuxR

Use

Synthetic Biology

Promoter

BBa_J23102

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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erCRY1b full length

Plasmid

#252023

Purpose

for ITC

Depositor

Joseph Takahashi

Article

Wickramaratne et al iScience. 2025 Nov 11;28(12):114015. doi

Insert

European robin cry 1b

Tags

His tag

Expression

Insect

Promoter

p10

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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erCRY1b crystal

Plasmid

#252022

Purpose

crystal structure

Depositor

Joseph Takahashi

Article

Wickramaratne et al iScience. 2025 Nov 11;28(12):114015. doi

Insert

European robin cry1b

Tags

His tag

Expression

Insect

Promoter

p10

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PJ23102R1LuxR_PCTR1E2Crimson

Plasmid

#250892

Purpose

LuxR expressed under BBa_J23102; E2-Crimson under PCT synthetic promoter; component of BNeuAS6; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

E2-Crimson
LuxR

Use

Synthetic Biology

Promoter

BBa_J23102 and PCT

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLLacO1_R1_EGFP

Plasmid

#250897

Purpose

EGFP expressed under PL LacO1 promoter; part of BNeu AS11; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLTetO1_R1_EGFP

Plasmid

#250899

Purpose

EGFP expressed under PL TetO1 promoter; part of BNeu AS12; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL TetO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PCTR1EGFP_PJ23102R1LuxR

Plasmid

#250885

Purpose

EGFP expressed under PCT synthetic promoter and LuxR expressed under BBa_J23102 promoter; component of BNeuAS3; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

EGFP
LuxR

Use

Synthetic Biology

Promoter

BBa_J23102 and PCT

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PHLT_R1_E2Crimson

Plasmid

#250886

Purpose

E2-Crimson expressed under PHLT synthetic promoter; component of BNeuAS4; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PHLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLT_R1_E2Crimson

Plasmid

#250882

Purpose

E2-Crimson expression under PLT synthetic promoter; part of BNeu AS1*; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PR_R1_E2Crimson

Plasmid

#250896

Purpose

E2-Crimson expressed under PR promoter; component of Bneu AS10; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PR

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PHT_R4_EGFP

Plasmid

#250889

Purpose

EGFP expressed under PHT synthetic promoter and regulated by RBS 'R4'; part of BNeuAS9; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PHT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pBR25 CFAP36-3C-mStayGold-2Strep crBSDr (C. reinhardtii)

Plasmid

#238226

Purpose

CRISPR donor plasmid for C-terminal tagging of CFAP36 and insertion of Blasticidin resistance (C. reinhardtii)

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36

Use

CRISPR; Donor

Tags

-3C-mStayGold-2Strep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pBR25 IFT70 [A331R, A335R] crSpecR (C. reinhardtii)

Plasmid

#238230

Purpose

CRISPR donor plasmid for C-terminal knock-in of IFT70 [A331R, A335R] and insertion of Spectinomycin resistance (C. reinhardtii)

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

IFT70 [A331R, A335R]

Use

CRISPR; Donor

Tags

None

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab676-HBEGF-2a-mWasabi-WPRE

Plasmid

#249029

Purpose

This plasmid expresses the mWasabi flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mWasabi

Use

CRISPR

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab639-HBEGF-2a-mRuby3-WPRE

Plasmid

#249030

Purpose

This plasmid expresses the mRuby3 flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mRuby3

Use

CRISPR

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab672-HBEGF-2a-mTagBFP2-WPRE

Plasmid

#249031

Purpose

This plasmid expresses the mTagBFP2 flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mTagBFP2

Use

CRISPR

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab821-pcDNA3.1-HALO-3xFLAG

Plasmid

#249007

Purpose

This plasmid expresses the C terminally 3x-FLAG tagged HALO protein sequences for pulldown assays

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

HALO

Tags

3x-FLAG-V5

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab965-PX459-BRCA1-Nterm-guide1

Plasmid

#249005

Purpose

This plasmid expresses the Cas9/gRNA that will target the HBEGF gene locus

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

Cas9

Use

CRISPR

Tags

3xFLAG

Promoter

chicken beta-actin promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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