We narrowed to 6,994 results for: ef1a

1. MLM3587

   Plasmid

   #43886

   Purpose

   Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NN (binds G)

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

   Insert

   TALE-activator VP64 Backbone
   

   Use

   Tale transcriptional activator expression vector

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha

   Available since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

   Plasmid

   #162810

   Purpose

   STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   BsmBI_Intein_DD
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS1_4x7SKPylT_EF1_Ub-*GFP

   Plasmid

   #162806

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled GFP

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   EF1_Ub-(N-TAG)GFP
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   GFP (Met1,Amber)

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL1103

   Plasmid

   #129526

   Purpose

   pDEF-SpCas9, No Target Control Vector for CRISPRi, PbEF1a promoter, 1st Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   dSpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   NLS, 1xHA

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL1315

   Plasmid

   #129527

   Purpose

   pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   dSpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   NLS, 1xHA

   Expression

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC2.4

   Plasmid

   #226557

   Purpose

   NATURA gene encoding plasmid

   Depositor

   Dave Wee

   Article

   Tommaso et al Molecular Therapy: Nucleic Acid (2025)

   Insert

   NATURA reporter gene
   

   Use

   Luciferase; Piggybac

   Tags

   EGFP and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTBL3328 PB-sfGFP-WPRE-target

   Plasmid

   #226676

   Purpose

   PiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   sfGFP-WPRE-site3target
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

8. pSL1419

   Plasmid

   #219778

   Purpose

   pDEF-TurboID-GFPmut2, negative control for cytosolic expression

   Depositor

   Scott Lindner

   Article

   Rios et al bioRxiv [Preprint]. 2024 Mar 16:2024.02.

   Insert

   TurboID/GFPmut2
   

   Use

   Plasmodium, rodent malaria parasites

   Tags

   Expression

   Mutation

   Promoter

   PbEF1alpha promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. aCE106

   Plasmid

   #204496

   Purpose

   Small molecule helper circuit

   Depositor

   Ron Weiss

   Article

   Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

   Inserts

   rtTA3
   PhIF-ABI
   VPR-Pyl1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   hEF1a

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FB407

    Plasmid

    #203628

    Purpose

    Promoter of the elongation factor 1-alpha gene from P. digitatum (PDIG_59570).

    Depositor

    Jose Marcos

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    Pef1A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C413-E19: attB4-mPol2p>-attB1r

    Plasmid

    #162971

    Purpose

    Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    murine RNA polymerase 2 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD

    Plasmid

    #162814

    Purpose

    STELLA plasmid for amber suppression of C-terminally labelled SMIM38

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    SMIM38(C-TAG)-Intein-DD
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    SMIM38(Ochre52,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI

    Plasmid

    #162803

    Purpose

    Control STELLA destination plasmid for N-terminally labelled proteins

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub(G75,76A)-BsmBI
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Ubiquitin (G75,76A)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAS1_4x7SKPylT_EF1_Ub-*SMIM38

    Plasmid

    #162808

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled SMIM38

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)SMIM38
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    SMIM38 (Met1, Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAS_4x7SKPylT_EF1_Ub-*PIGBOS

    Plasmid

    #162809

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled PIGBOS

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)PIGBOS
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    PIGBOS (Met1, Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMpGWB110

    Plasmid

    #68564

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMpGWB408

    Plasmid

    #68673

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. alpha7nACHR_Nacho_mCherry

    Plasmid

    #182939

    Purpose

    alpha7 nACHR expression in mammalian cell lines

    Depositor

    Robert Brenner

    Article

    Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

    Insert

    NACH7 (CHRNA7 Human)
    

    Use

    Synthesized

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-CT3G-CERP2-MG

    Plasmid

    #175503

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE EF1α dCas9-VP64

    Plasmid

    #50918

    Purpose

    Constitutive dCas9-VP64 lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMpGWB236

    Plasmid

    #68627

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHAGE EF1α dCas9-KRAB

    Plasmid

    #50919

    Purpose

    Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLVX-3XMyc-mTrim25

    Plasmid

    #51394

    Depositor

    Narry Kim

    Article

    Kwon et al Nat Struct Mol Biol. 2013 Sep;20(9):1122

    Insert

    Trim25 (Trim25 Mouse)
    

    Use

    Lentiviral

    Tags

    3XMyc

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMpGWB203

    Plasmid

    #68594

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMpGWB127

    Plasmid

    #68581

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BII-ChBtW-iresFUCCI

    Plasmid

    #133371

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Clover-Geminin-IRES-mKO2-Cdt1
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMpGWB313

    Plasmid

    #68641

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Glucocorticoid receptor (GR)

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PB-CT3G-ERP2-MG

    Plasmid

    #175501

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMpGWB113

    Plasmid

    #68567

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Glucocorticoid receptor (GR)

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMpGWB103

    Plasmid

    #68557

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge