We narrowed to 13,093 results for: CAR

1. pGEMT-blastR-p2a-v5-dL5

   Plasmid

   #161980

   Purpose

   homologous donor template for endogenous tagging

   Depositor

   Joanna Wysocka

   Article

   Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

   Insert

   blastR-p2a-v5-dL5-GSlinker
   

   Available Since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEMT-v5-dL5-p2a-blastR

   Plasmid

   #161981

   Purpose

   homologous donor template for endogenous tagging

   Depositor

   Joanna Wysocka

   Article

   Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

   Insert

   GSlinker-V5-dL5-P2A-blastR
   

   Available Since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEPQDKN0332

   Plasmid

   #162314

   Purpose

   Reporter plasmid for TNT SP6 Coupled Wheat Germ Extract System with detection via HiBiT

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   SP6prom-EO1-sfGFP-HiBiT
   

   Use

   Synthetic Biology

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPQD0CB0026

   Plasmid

   #162285

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEPQD0CB0246

   Plasmid

   #162286

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEPQDKN0329

   Plasmid

   #162315

   Purpose

   TEV protease plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   T7prom-RBS-NET-TEVprotease-HiBiT
   

   Use

   Synthetic Biology

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEPQD0CM0027

   Plasmid

   #162309

   Purpose

   Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   C-terminal tag, 9 aa linker, superfolder GFP, strep tag, stop codon
   

   Use

   Synthetic Biology

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEPQD0CM0549

   Plasmid

   #162300

   Purpose

   Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   N-terminal tag, SUMO-[TEV cleavage site]
   

   Use

   Synthetic Biology

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEPQD0CM0550

   Plasmid

   #162301

   Purpose

   Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   N-terminal tag, HiBiT-SUMO-[TEV cleavage site]
   

   Use

   Synthetic Biology

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEPQD0KN0283

    Plasmid

    #162288

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEPQD0KN0282

    Plasmid

    #162287

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEPQD0KN0244

    Plasmid

    #162282

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPQD0KN0024

    Plasmid

    #162281

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPQD0CM0539

    Plasmid

    #162306

    Purpose

    Level 0 CDS part for MoClo assembly, AATG-TTCG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    CDS, TEV protease (S219V), no stop codon
    

    Use

    Synthetic Biology

    Mutation

    S219V, codon optimized for E. coli

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEPMY0SP0002

    Plasmid

    #162304

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, 6xHis tag-[HRV 3C cleavage site]
    

    Use

    Synthetic Biology

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPQD0CM0551

    Plasmid

    #162303

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEPQD0CM0547

    Plasmid

    #162298

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, TrxA-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEPQD0CM0546

    Plasmid

    #162297

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, MBP-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEPQD0CM0544

    Plasmid

    #162295

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, MBP-[Factor Xa cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEPQD0CM0543

    Plasmid

    #162294

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, GST-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge