We narrowed to 11,275 results for: Vars;

1. pDONR223_TBK1_WT

   Plasmid

   #82285

   Purpose

   Gateway Donor vector containing TBK1, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Gateway entry vector

   Mutation

   N388D; K570Q

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139097

   Purpose

   Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dxCas9-NG
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223_REG1A_WT

   Plasmid

   #81785

   Purpose

   Gateway Donor vector containing REG1A , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   REG1A (REG1A Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223 SARS-CoV-2 ORF9B_nostop

   Plasmid

   #149327

   Purpose

   Gateway-compatible Entry vector, with insert of ORF9B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF9B
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pDONR223_SMAD3_WT

   Plasmid

   #82272

   Purpose

   Gateway Donor vector containing SMAD3, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SMAD3 (SMAD3 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR223 SARS-CoV-2 ORF9Bwu

   Plasmid

   #149331

   Purpose

   Gateway-compatible Entry vector, with insert of ORF9Bwu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF9Bwu
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pDONR223_NFKBIA_WT

   Plasmid

   #81833

   Purpose

   Gateway Donor vector containing NFKBIA ,part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NFKBIA (NFKBIA Human)
   

   Use

   Gateway entry vector

   Mutation

   S76P

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223_U2AF1_WT

   Plasmid

   #81954

   Purpose

   Gateway Donor vector containing U2AF1, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   U2AF1 (U2AF1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-CARD14-E138A

   Plasmid

   #69921

   Purpose

   Expresses mutant FLAG-CARD14 in mammalian cells

   Depositor

   Francesca Capon

   Article

   Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

   Insert

   CARD14 isoform 3 (CARD14 Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   E138A

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pDONR223 SARS-CoV2 TEV-NSP12

    Plasmid

    #154410

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid

    #106183

    Purpose

    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-Venus-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

    Promoter

    hSynapsin

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_PIK3CG_WT

    Plasmid

    #81843

    Purpose

    Gateway Donor vector containing PIK3CG , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PIK3CG (PIK3CG Human)
    

    Use

    Gateway entry vector

    Mutation

    E2G

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223_PRDM1_WT

    Plasmid

    #81761

    Purpose

    Gateway Donor vector containing PRDM1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PRDM1 (PRDM1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223_OMA1_WT

    Plasmid

    #81889

    Purpose

    Gateway Donor vector containing OMA1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    OMA1 (OMA1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223_TSC2_WT

    Plasmid

    #82281

    Purpose

    Gateway Donor vector containing TSC2, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TSC2 (TSC2 Human)
    

    Use

    Gateway entry vector

    Mutation

    A210V; K704E; L1015M; I1649M

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

    Plasmid

    #169336

    Purpose

    Expression of the LT-Fucci(SA) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Fucci(SA) (GMNN, CDT1 Human)
    

    Tags

    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223_U2AF1_p.S34F

    Plasmid

    #81358

    Purpose

    Gateway Donor vector containing U2AF1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Gateway entry vector

    Mutation

    S34F

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223_POT1_p.G76V

    Plasmid

    #81497

    Purpose

    Gateway Donor vector containing POT1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    POT1 (POT1 Human)
    

    Use

    Gateway entry vector

    Mutation

    G76V

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223 SARS-CoV2 TEV-NSP5

    Plasmid

    #154404

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pDONR223 SARS-CoV2 TEV-S

    Plasmid

    #154415

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of s…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    Sept. 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. p1.1-mCherry

    Plasmid

    #162772

    Purpose

    Fluorescent reporter for CHO expression studies

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Insert

    red fluorescent protein
    

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p1.2-GS-eGFP

    Plasmid

    #162771

    Purpose

    Fluorescent reporter for CHO expression studies

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Insert

    enhanced green fluorescent protein
    

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDONR223_POT1_p.L388M

    Plasmid

    #81570

    Purpose

    Gateway Donor vector containing POT1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    POT1 (POT1 Human)
    

    Use

    Gateway entry vector

    Mutation

    L388M

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDONR223_POT1_p.L265H

    Plasmid

    #81559

    Purpose

    Gateway Donor vector containing POT1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    POT1 (POT1 Human)
    

    Use

    Gateway entry vector

    Mutation

    L265H

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMXS-IRES-Blast POLE1 N-ISCmut

    Plasmid

    #160803

    Purpose

    Express N-terminal ISC mutant POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 N-ISCmut (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBridge-tyrosinase.σ1A

    Plasmid

    #197381

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-1 σ1A
    

    Tags

    GAL4-DNA binding domain fragment, HA tag, and SV4…

    Expression

    Yeast

    Mutation

    Contains an extra 42 nucleotides encoding 14 resi…

    Promoter

    ADH1 and MET25

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR223 SARS-CoV2 TEV-NSP1

    Plasmid

    #154400

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pDECKO_GFP_AAVS1

    Plasmid

    #141211

    Purpose

    pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Insert

    gRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR223_VEGFC_WT

    Plasmid

    #82282

    Purpose

    Gateway Donor vector containing VEGFC, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    VEGFC (VEGFC Human)
    

    Use

    Gateway entry vector

    Mutation

    K414Q

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-CARD14-G117S

    Plasmid

    #69920

    Purpose

    Expresses mutant FLAG-CARD14 in mammalian cells

    Depositor

    Francesca Capon

    Article

    Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    G117S

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only