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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,326 results for: PUC19

Showing: 2221 - 2230 of 2326 results

  1. pGLOW39

    Plasmid
    #173068
    Purpose
    mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms
    Depositor
    Ryan Doonan
    Article
    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791
    Insert
    mScarlet-I-C1^SEC^3xMyc
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized mScarlet
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    173068-plasmid-map-sequence-id-345944

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  2. pACT1:Cas9-GFP, U6:sgTK

    Plasmid
    #122852
    Purpose
    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene
    Depositor
    David Sibley
    Article
    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19
    Inserts
    Cas9-GFP
    U6-sgTK
    Use
    CRISPR; Cas9-gfp plasmid for genome editing of cr…
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    Cryptosporidium parvum U6 and Cryptosporidium par…
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    122852-plasmid-map-sequence-id-238880

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  3. pACT1:Cas9-GFP, U6:sgUPRT

    Plasmid
    #122853
    Purpose
    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene
    Depositor
    David Sibley
    Article
    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19
    Inserts
    Cas9-GFP
    U6-sgUPRT
    Use
    CRISPR; Cas9-gfp plasmid for genome editing of cr…
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    Cryptosporidium parvum U6 and Cryptosporidium par…
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    122853-plasmid-map-sequence-id-238877

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  4. pJW1583

    Plasmid
    #121054
    Purpose
    GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    GFP^SEC^AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, C. elegans codon optimized GFP, and auxin…
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    June 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    121054-plasmid-map-sequence-id-235990

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  5. pJW1584

    Plasmid
    #121055
    Purpose
    YPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    YPET^SEC^AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, C. elegans codon optimized YPET, and auxi…
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    June 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    121055-plasmid-map-sequence-id-235991

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  8. UPRT-mCh-Nluc-P2A-neo-UPRT

    Plasmid
    #135015
    Purpose
    Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus
    Depositor
    David Sibley
    Article
    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19
    Inserts
    mCh-Nluc-P2A-neo
    UPRT 5'UTR
    UPRT 3'UTR
    Use
    Cryptosporidium expression
    Tags
    mCherry and Nluc-neo
    Expression
    Mutation
    Promoter
    Cryptosporidium actin and enolase
    Available Since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135015-plasmid-map-sequence-id-266476

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  9. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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Showing: 2221 - 2230 of 2326 results