We narrowed to 6,471 results for: poly

1. PB-Neo-TetO-Cdk12_Untagged

   Plasmid

   #127178

   Purpose

   Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Piggybac transposase vector

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   TetO Promoter

   Available since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Frt-V5-hspB8

   Plasmid

   #63109

   Purpose

   Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb8 (HSPB8 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR8-PHF19

   Plasmid

   #68864

   Purpose

   GW entry vector for PHF19

   Depositor

   Adrian Bracken

   Article

   Brien et al Nat Struct Mol Biol. 2012 Dec;19(12):127

   Insert

   PHF19 (PHF19 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

4. Frt-hspB5

   Plasmid

   #63096

   Purpose

   Expression of HSPB5 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb5 (CRYAB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. hRAD18 dC2-EGFP

   Plasmid

   #68826

   Purpose

   Expresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP

   Depositor

   Satoshi Tateishi

   Article

   Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

   Insert

   human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pPB_Cdc42-TomKat-sensor-CAAX

   Plasmid

   #172473

   Purpose

   Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.

   Depositor

   Sean R Collins

   Article

   Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

   Insert

   tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
   

   Use

   Piggybac transposon

   Tags

   polybasic and CAAX motif from Kras, tdKatushka2, …

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. W118-1_Flag-hIRAK1

   Plasmid

   #180405

   Purpose

   lentiviral transduction of human IRAK1 (with an N-terminal Flag tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   IRAK1 (IRAK1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-UbC-mEmerald Rab7a

   Plasmid

   #115238

   Purpose

   To express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.

   Depositor

   Pantelis Tsoulfas

   Insert

   Rab7a fused to mEmerald (RAB7A Human)
   

   Use

   AAV

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   7 Amino Acids (21 Nucleotides) between mEmerald a…

   Promoter

   Human UbC

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET19-MTdTc302

   Plasmid

   #207458

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   MTdTc302 (Dntt Mouse)
   

   Use

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry

    Plasmid

    #221081

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST

    Plasmid

    #42650

    Purpose

    destination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 299 SNP (TLR4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D299G

    Promoter

    CMV/TO

    Available since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Cdt1 human

    Plasmid

    #72681

    Purpose

    mammalian expression of human Cdt1

    Depositor

    Domenico Maiorano

    Article

    Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

    Insert

    Cdt1 (CDT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

    Plasmid

    #63139

    Purpose

    Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

    Depositor

    Titia Sixma

    Article

    Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

    Inserts

    Ring1b (Ring1 Mouse)
    Bmi1 (Bmi1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-109 and Residues 1-159

    Promoter

    tac

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pGV3319

    Plasmid

    #170277

    Purpose

    Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    Bst-LF D720A
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    D720A

    Promoter

    T7-lac

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEM-HSPA5P

    Plasmid

    #44150

    Purpose

    a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

    Depositor

    Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    HSPA5 promoter (HSPA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pGEX-6P-1_WT RAD23A

    Plasmid

    #201456

    Purpose

    Expresses human RAD23A with an N-terminal GST tag in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    tac promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Frt-hspB1

    Plasmid

    #63092

    Purpose

    Expression of HSP1 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb1 (HSPB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET32a-HD39Q

    Plasmid

    #11514

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 39 glutamines (HTT Human)
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 39 glutamines, vector m…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid

    #138525

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    Control non-PRC2 binding A-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJ4M/TDP-43 S48E

    Plasmid

    #104481

    Purpose

    bacterial expression of TDP-43 S48E

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    S48E

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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