We narrowed to 42,752 results for: INA

1. pLJM1-Flag-Rheb-4Rs

   Plasmid

   #165022

   Purpose

   expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)

   Depositor

   Ken Inoki

   Article

   Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

   Insert

   Rheb-4Rs (RHEB Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   change lysine residues on position 109, 135, 151,…

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-DHFR F[1,2]-N (TRP1)

   Plasmid

   #177797

   Purpose

   Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Tags

   DHFR F[1,2]

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFIN-RK-GFP-WPRE

   Plasmid

   #44358

   Depositor

   Susan Semple-Rowland

   Article

   Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

   Insert

   RK-GFP (MAPK14 Synthetic, Human)
   

   Use

   Lentiviral

   Promoter

   human rhodopsin kinase (pos 2557-2853)

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-Jun4A-Myc

   Plasmid

   #47444

   Purpose

   mammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)

   Depositor

   Axel Behrens

   Article

   Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

   Insert

   Jun 4A mutant (Jun Mouse)
   

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   four N-terminal residues phosphorylated by JNK, s…

   Promoter

   CMV

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. px330 Rosa26 AscI LH291Gu1 (LH500)

   Plasmid

   #99628

   Purpose

   Plasmid used to target the pre-modified Rosa26 allele with Cas9

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiRCas9-Lambda2

   Plasmid

   #104184

   Purpose

   Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

   Depositor

   Eugene Yeo

   Article

   Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

   Insert

   U6-sgRNA, EFS-PIN-dCas9
   

   Use

   CRISPR and Lentiviral

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-Ikka-HA (K44M)

   Plasmid

   #23297

   Depositor

   Warner Greene

   Article

   Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

   Insert

   IKK alpha (Chuk Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K44M

   Available Since

   April 12, 2010

   Availability

   Academic Institutions and Nonprofits only

8. px330 Rosa26 Gu1 (LH416)

   Plasmid

   #99629

   Purpose

   Plasmid used to target the wildtype Rosa26 allele with Cas9

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-IpgD(C439S)

   Plasmid

   #183667

   Purpose

   N-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   IpgD
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C439S

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV_PP1-mCitrine

    Plasmid

    #172472

    Purpose

    Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

    Depositor

    Sean R Collins

    Article

    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

    Insert

    prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
    

    Use

    Lentiviral

    Tags

    mCitrine and prolactin signal sequence (cleaved p…

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pS6-hα2M-H6

    Plasmid

    #128495

    Purpose

    Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (G27-S1468) (A2M Human)
    

    Tags

    His tag (6x)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEMS2281

    Plasmid

    #111902

    Purpose

    ssAAV genome with Ple346 (NEFM MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple346 (NEFM Human)
    

    Use

    AAV

    Promoter

    Ple346 (NEFM MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLINK cyclin T1 (1-280) myc (K93D) (P#580)

    Plasmid

    #14611

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-280) K93D (CCNT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K93D. Deficient in binding to CDK9. Inactive in 3…

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA5-LAP-MPS1-KD

    Plasmid

    #114045

    Purpose

    Expression of exogenous LAP tagged MPS1 Kinase-dead

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    MPS1-KD (TTK Human)
    

    Expression

    Mammalian

    Mutation

    D664A kinase dead

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. LoxP2-Mb2Tomato-2A (SO240)

    Plasmid

    #99614

    Purpose

    To clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2Tomato
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIE-hα2M-H6

    Plasmid

    #128492

    Purpose

    Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (A2M Human)
    

    Tags

    His tag (6x)

    Expression

    Insect

    Promoter

    IE1

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LoxP2-H2B-EGFP-V5-2A (SO107)

    Plasmid

    #99617

    Purpose

    To clone gene of interest downstream of LoxP2-H2B-EGFP-V5-2A

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    H2B-EGFP-V5
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. LoxP1-Mb2YFP-2A (SO244)

    Plasmid

    #99613

    Purpose

    To clone gene of interest downstream of LoxP1-Mb2YFP-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2EYFP
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. LoxP3-HA-H2B-Cerulean-2A (SO104)

    Plasmid

    #99618

    Purpose

    To clone gene of interest downstream of LoxP3-HA-H2B-Cerulean-2A

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    HA-H2B-Cerulean
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR3-FLAG-Gephyrin GC

    Plasmid

    #68818

    Purpose

    Flag tagged mammalian expression of GC domain

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    NIL

    Promoter

    CMV

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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