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pB-Neo-DEST (JDW 940)

Plasmid

#229825

Purpose

A neo selectable, PiggyBac destination vector compatible with gateway cloning system.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-F14F15S-jGCaMP7s

Plasmid

#178514

Purpose

AAV vector for Flpo recombinase dependent jGCaMP7s

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

jGCaMP7s

Use

AAV

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-mCre

Plasmid

#178515

Purpose

AAV vector for mCre recombinase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mCre

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_005

Plasmid

#180555

Purpose

Entry vector containing Terminator sequence (Bba_B0015)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator sequence (Bba_B0015)

Expression

Bacterial

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_047

Plasmid

#180551

Purpose

Entry vector containing sfGFP in E1.3a format

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sfGFP in E1.3a format

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_007

Plasmid

#180563

Purpose

Entry vector containing Terminator sequence (Bba_B0015)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator sequence (Bba_B0015)

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_044

Plasmid

#180532

Purpose

Entry vector containing J23117 anderson promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23117 anderson promoter

Expression

Bacterial

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_043

Plasmid

#180531

Purpose

Entry vector containing J23101 anderson promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23101 anderson promoter

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_031

Plasmid

#180556

Purpose

Entry vector containing Terminator - BBa_B0010 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator - BBa_B0010 terminator

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_032

Plasmid

#180528

Purpose

Entry vector containing pLac promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pLac promoter

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_252

Plasmid

#180553

Purpose

Entry vector containing mScarlet in E1.3b format

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

mScarlet in E1.3b format

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_042

Plasmid

#180530

Purpose

Entry vector containing J23100 anderson promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23100 anderson promoter

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-Squash-SAM-sensor 4-2

Plasmid

#177916

Purpose

Expresses Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-Squash-SAM-sensor 4-2

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET-QUE7mu R122A

Plasmid

#129317

Purpose

mutant of QUEEN-7+D20

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-7mu R122A

Expression

Bacterial

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pgRNA_CV2

Plasmid

#167165

Purpose

pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.

Depositor

Alexander Loewer

Article

Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMZ039

Plasmid

#67757

Purpose

Expresses N-terminal kinase domain of Chk1 with C-term GFP fluorescent tag

Depositor

Michael Lampson

Article

Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

Insert

zebrafish Chk1

Tags

GFP

Mutation

only encodes N-term kinase domain (amino acids 1-…

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-rACh1l

Plasmid

#234414

Purpose

Expresses the red ACh sensor GRAB rACh1l in neurons

Depositor

Yulong Li

Article

Xie et al Nat Neurosci. 2026 Jun 16. doi: 10.1038/

Insert

GRAB-rACh1l

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 23, 2026

Availability

Academic Institutions and Nonprofits only

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pDM1514-Plin

Plasmid

#252025

Purpose

Dictyostelium discoideum, safe haven knock-in plasmid for the act5 locus, N-terminal mCherry tagging

Depositor

Thierry Soldati

Article

Perret et al Sci Adv. 2026 Jan 2;12(1):eady0812. doi:

Insert

plin

Use

Amoebal expression

Tags

mCherry

Available Since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pDM1513-Vps32

Plasmid

#252024

Purpose

Dictyostelium discoideum, safe haven knock-in plasmid for the act5 locus, N-terminal GFP tagging of the gene Vps32

Depositor

Thierry Soldati

Article

Perret et al Sci Adv. 2026 Jan 2;12(1):eady0812. doi:

Insert

Vps32

Use

Amoebal expression

Tags

GFP

Available Since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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knockin GAPDH-GvpNtoV

Plasmid

#232726

Purpose

GvpN to GvpV genes knockin at the GAPDH locus

Depositor

Xiaojun Lian

Article

Kim et al BMC Biotechnol. 2026 Apr 30. doi: 10.118

Insert

GvpN to GvpV

Expression

Mammalian

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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