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pCK948

Plasmid

#242917

Purpose

Constitutive expression of dCas9, MCP-TetD, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKO025

Plasmid

#242913

Purpose

aTc-inducible expression of Spy-dCas9-AsiA and Sth1-dCas9, and constitutive expression of MCP-SoxS

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

Sth1-dCas9

Use

CRISPR and Synthetic Biology

Promoter

Ptet

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCK660

Plasmid

#242914

Purpose

Constitutive expression of dCas9, MCP-SoxS, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK320

Plasmid

#242906

Purpose

aTc-inducible expression of dCas9-AsiA

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

AsiA (asiA Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

TetR-Ptet

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-aex-5-flag-tev-halo-his

Plasmid

#227152

Purpose

For T-REX experiments of aex-5.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

aex-5 (aex-5 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-asp-2-flag-tev-halo-his

Plasmid

#227153

Purpose

For T-REX experiments of asp-2.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

asp-2 (asp-2 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-23012

Plasmid

#109180

Purpose

Plasmid for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-23122

Plasmid

#109181

Purpose

Plasmid for expression of N-terminally 3xFLAG-tagged proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Tags

3xFLAG

Expression

Insect

Promoter

minimal HSP70 promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacD-23003

Plasmid

#109183

Purpose

Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacD-23004

Plasmid

#109184

Purpose

Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacD-23005

Plasmid

#109185

Purpose

Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-21122

Plasmid

#109174

Purpose

Plasmid for expression of N-terminally 3xFLAG-tagged proteins under the control of a polh promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Tags

3xFLAG

Expression

Insect

Promoter

polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-21222

Plasmid

#109175

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged proteins under the control of a polh promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Tags

3xFLAG

Expression

Insect

Promoter

polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb827

Plasmid

#217500

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb837

Plasmid

#217501

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb847

Plasmid

#217502

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb84 backbone, pRO1600/ColE1 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb867

Plasmid

#217503

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb86 backbone, p15A ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb887

Plasmid

#217504

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb88 backbone, pUC ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb897

Plasmid

#217505

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-EGFP-V5

Plasmid

#240240

Purpose

Expression of EGFP-V5 under control of copper-inducible promoter.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

EGFP-V5

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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