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pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-1

Plasmid

#200529

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-2

Plasmid

#200530

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_T2A-mCherry-P2A-NTRv2-pA ORF-3

Plasmid

#200531

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-BFP-pA ORF-1 without ATG

Plasmid

#200534

Purpose

mini circle middle entry plasmid for 2a-BFP-pA ORF 1 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-BFP-pA ORF-2 without ATG

Plasmid

#200535

Purpose

mini circle middle entry plasmid for 2a-BFP-pA ORF 2 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-ME_2a-BFP-pA ORF-3 without ATG

Plasmid

#200536

Purpose

mini circle middle entry plasmid for 2a-BFP-pA ORF 3 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13198

Plasmid

#219873

Purpose

The base plasmid of TUNEYALI forTF08

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF08 ( YALI1_D33640g) and homologous arm matching TF08

Expression

Yeast

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13236

Plasmid

#219909

Purpose

The base plasmid of TUNEYALI for TF46

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF46 ( YALI1_E20229g) and homologous arm matching TF46

Expression

Yeast

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDL655

Plasmid

#231165

Purpose

T-DNA encoding gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

gRNA targeting SlPDS

Expression

Plant

Promoter

AtU6

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13199

Plasmid

#219874

Purpose

The base plasmid of TUNEYALI forTF09

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF09 (YALI1_F29652g) and homologous arm matching TF09

Expression

Yeast

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13218

Plasmid

#219893

Purpose

The base plasmid of TUNEYALI for TF28

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF28 (YALI1_B28150g) and homologous arm matching TF28

Expression

Yeast

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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EYFP-Parkin

Plasmid

#210292

Purpose

mitophagy marker

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

EYFP-Parkin

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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EYFP-FKBP-N-WASP

Plasmid

#210302

Purpose

targeted actin nucleation

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

EYFP-FKBP-N-WASP

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13239

Plasmid

#219912

Purpose

The base plasmid of TUNEYALI for TF49

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49

Expression

Yeast

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13247

Plasmid

#219918

Purpose

The base plasmid of TUNEYALI for TF57

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF57 (YALI1_D17249g) and homologous arm matching TF57

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13228

Plasmid

#219903

Purpose

The base plasmid of TUNEYALI for TF38

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF38 (YALI1_A14345g) and homologous arm matching TF38

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13210

Plasmid

#219885

Purpose

The base plasmid of TUNEYALI for TF20

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF20 (YALI1_D16173g) and homologous arm matching TF20

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-miniCGBE1

Plasmid

#198548

Purpose

Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-miniCGBE1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRRH

Plasmid

#198540

Purpose

Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRRH

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRCH

Plasmid

#198542

Purpose

Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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