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pcDNA3. Flag-CSL WT

Plasmid

#167004

Purpose

Mammalian expression of CSL WT

Depositor

Gian-Paolo Dotto

Publication

Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CSL (RBPJ Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFastbac1-Flag-FANCD2

Plasmid

#134904

Purpose

codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

Depositor

Andrew Deans

Publication

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

FANCD2 (FANCD2 Human)

Tags

Flag

Expression

Insect

Mutation

fully synthetic, codon optimized for insect expre…

Promoter

polyhedron

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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Brm WT-Flag pShuttle

Plasmid

#122105

Purpose

Expression of full length Brm in mammalian cells

Depositor

Paul Herring

Publication

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BRM (SMARCA2 Human)

Tags

Flag

Expression

Mammalian

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-mPer2ER-pBABEpuro

Plasmid

#61430

Purpose

Express mPER2-ER* fusion protein

Depositor

Aziz Sancar

Publication

Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

Insert

mPER2 (Per2 Mouse)

Use

Retroviral

Tags

Flag and mouse estrogen receptor ligand binding d…

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pWPI-FLAG-hPRKCI

Plasmid

#35387

Depositor

Luke McCaffrey

Insert

Protein Kinase C iota (PRKCI Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available since

June 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pRK5-Merlin-Flag-HA

Plasmid

#27103

Depositor

Filippo Giancotti

Publication

Li et al Cell. 2010 Feb 19. 140(4):477-90.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Merlin (Nf2 Mouse)

Tags

Flag and HA

Expression

Mammalian

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pBABE HSF Flag Ac

Plasmid

#1949

Depositor

Robert Kingston

Publication

Sullivan et al Mol Cell Biol 2001 Sep;21(17):5826-37.

Insert

Heat Shock Factor 1 (HSF1 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

Acidic mutations (D416, E493, E496 A)

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pFastBac mph1 flag

Plasmid

#1985

Depositor

Robert Kingston

Publication

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

mPh1 (Phc1 Mouse)

Tags

flag

Expression

Insect

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pSLIK_neo_3xflag_CHEK2

Plasmid

#136536

Purpose

Lentiviral expression vector for an inducible 3xflag-tagged CHEK2

Depositor

Kevin Janes

Publication

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

CHEK2 (CHEK2 Human)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

3x FLAG

Expression

Mammalian

Available since

June 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET-Duet-ECHA(37-763)-FLAG (no His-tag)

Plasmid

#96887

Purpose

expresses flag-tagged mouse ECHA (37-768) in E.coli cells (no His-tag)

Depositor

Hening Lin

Publication

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)

Tags

FLAG-tagged

Expression

Bacterial

Mutation

deleted amino acid 1-36

Promoter

T7

Available since

July 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-EcSTH-FLAG

Plasmid

#218628

Purpose

This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

Depositor

Valentin Cracan

Publication

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

EcSTH

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a-Ronin-FLAG-IRES-Neo

Plasmid

#28020

Depositor

Thomas Zwaka

Publication

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ronin (THAP11 Human)

Tags

FLAG

Expression

Mammalian

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pWZL Neo Myr Flag NTRK3

Plasmid

#20551

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

NTRK3 (NTRK3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CHEK1

Plasmid

#20455

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CHEK1 (CHEK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pBabe-Puro-Myr-Flag-IKBKE

Plasmid

#15293

Depositor

William Hahn

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKBKE (IKBKE Human)

Use

Retroviral

Tags

Myr-Flag

Expression

Mammalian

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pWZL-blast Myr-FLAG-p110alpha

Plasmid

#10889

Depositor

William Hahn

Publication

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

p110alpha (PIK3CA Bovine)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
3XFLAG_ALDOA in pCW57_tTA_Blast

Plasmid

#154210

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of ALDOA

Depositor

David Sabatini

Publication

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

ALDOA (ALDOA Human)

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Promoter

TRE repeats (dox off)

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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GAL FLAG25QDProCFPp303

Plasmid

#15584

Depositor

Susan Lindquist

Publication

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pHAGE Flag HA ADAR1 p150

Plasmid

#237855

Purpose

Lentiviral expression of ADAR1 p150

Depositor

David Pellman

Publication

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

ADAR1 (ADAR Human)

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK-Rbfox2-rABE-Flag

Plasmid

#191384

Purpose

Doxycycline inducible expression of Rbfox2-rABE

Depositor

Stephen Floor

Publication

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

Rbfox2-rABE (RBFOX2 Synthetic, Human)

Use

Lentiviral

Tags

Flag

Promoter

tet-inducible promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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