We narrowed to 6,471 results for: poly

1. pX330_gRNA_H19-DMR-1

   Plasmid

   #159929

   Purpose

   Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse H19-DMR (H19-icr Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDF11-SFPQ(214-598)_quadruple_mutant

   Plasmid

   #135441

   Purpose

   Expresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tag

   Depositor

   Charles Bond

   Article

   Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

   Insert

   splicing factor proline and glutamine rich (SFPQ Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   L535A, L539A, L546A, M549A

   Promoter

   T7

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBacMam2-DiEx-LIC-N entry vector

   Plasmid

   #111751

   Purpose

   Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N1 Dynamin1 T78R L84R T92R V118R

   Plasmid

   #112106

   Purpose

   Mammalian expression plasmid of GFP-tagged Dynamin protein.

   Depositor

   Justin Taraska

   Article

   Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

   Insert

   Dynamin1 (DNM1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T78R L84R T92R V118R

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. hASXL(1-115)-luciferase

   Plasmid

   #106427

   Purpose

   Expresses human ASXL1 (amino acids 1-115) fused to the N-terminal of firefly luciferase

   Depositor

   Douglas Feldman

   Article

   Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

   Insert

   addition sex combs like 1 ASXL1 (ASXL1 Human)
   

   Use

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   amino acids 1-115 fused to luciferase

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJC20cdc8.27

   Plasmid

   #99362

   Purpose

   Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Glutamic acid 129 to Lysine

   Promoter

   T7

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-GST-CHD5-WT-FRAG

   Plasmid

   #68873

   Purpose

   IPTG inducible bacterial expression of CHD5 fragment

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5 (CHD5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   331-672aa

   Promoter

   tac

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCR2.1-Ins2-842

   Plasmid

   #53969

   Purpose

   842 bp insert from mouse Ins2 gene used as a control for methylation-specific PCR assays

   Depositor

   Kevin Ferreri

   Article

   Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

   Insert

   mouse Ins2 gene partial (Ins2 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMHE-mScarlet-hCenpC

   Plasmid

   #171490

   Purpose

   T7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   CenpC (CENPC Human)
   

   Use

   Pgehme

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

    Plasmid

    #107581

    Purpose

    B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pdh promoter Bacillus megaterium DSM319

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PCRII apoEb

    Plasmid

    #121901

    Purpose

    Plasmid for making zebrafish apoEb in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    Apoeb (apoeb Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-DN-RFX

    Plasmid

    #52296

    Purpose

    Dominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    Dominant negative RFX
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. PCRII apoEa

    Plasmid

    #121900

    Purpose

    Plasmid for making zebrafish apoEa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoEa (apoea Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Str-KDEL_SBP-EGFP-CCR5-Cys3A

    Plasmid

    #222313

    Purpose

    Synchronize the trafficking of CCR5-Cys3A from the ER.

    Depositor

    Franck Perez

    Article

    Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

    Insert

    Streptavidin-KDEL and CCR5-Cys3A fused to SBP-EGFP (CCR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C321A, C323A, C324A

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-mClover3-bElys

    Plasmid

    #171494

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged bovine Elys mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Elys (AHCTF1 B. tarus (bovine))
    

    Use

    Pgehme

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. p1A Prk-

    Plasmid

    #162694

    Purpose

    p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Prk inactive (K20M S21A native protein, this map …

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PCRII apoBa

    Plasmid

    #121897

    Purpose

    Plasmid for making zebrafish apoBa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoBa (apoba Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. W118-1_hTGM1-Flag

    Plasmid

    #180406

    Purpose

    lentiviral transduction of human TGM1 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM1 (TGM1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21b(+)-Is-PETase

    Plasmid

    #112202

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

    Depositor

    Christopher Johnson, Gregg Beckham

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid

    #208942

    Purpose

    The CE1 construct, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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