We narrowed to 6,928 results for: 11

1. API5_pLX307

   Plasmid

   #98315

   Purpose

   Lentiviral expression of API5

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   API5 (API5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-nAChr-Alpha6-YFP

   Plasmid

   #50483

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Use

   Tags

   YFP fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Mutation

   Gly-Ala-Gly flexible linker flanking the YFP open…

   Promoter

   CMV

   Available since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pGEX4T-1 Slp2-a E11A/R32A

   Plasmid

   #40063

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   Slp2-a E11A/R32A (Sytl2 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Glutamate 11 to Alanine, Arginine 32 to Alanine

   Promoter

   Tac

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pEGFP-C1 Slp2-a E11A/R32A

   Plasmid

   #40055

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   Slp2-a E11A/R32A (Sytl2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Glutamate 11 to Alanine, Arginine 32 to Alanine

   Promoter

   CMV promoter

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pLentiCRISPR v2 puro mGSDME gRNA2

   Plasmid

   #223522

   Purpose

   Knocking out mGSDME in mouse cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (GSDME Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiCRISPR v2 puro mGSDME gRNA1

   Plasmid

   #223521

   Purpose

   Knocking out mGSDME in mouse cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (GSDME Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCIneo-lambdaN-HA-HsEDC4-del974-1265_V

   Plasmid

   #147786

   Purpose

   Mammalian Expression of HsEDC4-del974-1265

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsEDC4-del974-1265 (EDC4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation I286T compared to the seq…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pD649-HAsp-PSG11-COMP5AP-AviTag-9xHis

   Plasmid

   #157304

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PSG11 (PSG11 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pD649-HAsp-PSG9-COMP5AP-AviTag-9xHis

   Plasmid

   #157388

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PSG9 (PSG9 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. W118-1_eGFP

    Plasmid

    #180379

    Purpose

    lentiviral transduction of eGFP gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    eGFP (eGFP )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. His-MBP-IST1 N-terminal domain

    Plasmid

    #184805

    Purpose

    Bacterial expression of IST1 N-terminal domain

    Depositor

    James Hurley

    Article

    Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    aa 1-187 only

    Promoter

    T7

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)

    Plasmid

    #185717

    Purpose

    AAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    synuclein, alpha (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Changed Ala 11 to Pro, Val 70 to Pro

    Promoter

    hSyn

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. His-MBP-CHMP1B, S2C

    Plasmid

    #184803

    Purpose

    Bacterial expression of CHMP1B (S2C mutation)

    Depositor

    James Hurley

    Article

    Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    S2C

    Promoter

    T7

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-2T-CBP(1603-2678)

    Plasmid

    #183774

    Purpose

    Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

    Insert

    nej (nej Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSUMO GLTP

    Plasmid

    #170732

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

    Insert

    GLTP (GLTP Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. sgRNA targeting human SLC12A5 gene stop codon

    Plasmid

    #158577

    Purpose

    For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    sgRNA targeting human SLC12A5 gene stop codon
    

    Use

    CRISPR

    Tags

    Cas9 and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cry2 mcherry Gamma 9 in pcDNA3.1

    Plasmid

    #64208

    Purpose

    mCherry fused between Cry2 and gamma 9 to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 (CRY2 Mustard Weed)
    gamma cone (GNGT2 Human)
    

    Use

    Tags

    mcherry and n/a

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid

    #50482

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    CFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the CFP open…

    Promoter

    CMV

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pSAM2_mCherry_Gata6

    Plasmid

    #72694

    Purpose

    Doxycyline regulated expression of Gata6 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Gata 6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUW-TetON-Sox10

    Plasmid

    #115242

    Purpose

    Lentiviral plasmid for tet-inducible expression of Sox10 (generated from plasmid #20321)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA3.1-nAChr-beta3-YFP

    Plasmid

    #50484

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    YFP fusion in M3-M4 loop (after residue P379). O…

    Promoter

    CMV

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

22. Lenti-117G-hyeA3A-BE4max

    Plasmid

    #157946

    Purpose

    Lentiviral expression of hyeA3A-BE4max

    Depositor

    Dali Li

    Article

    Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

    Insert

    Lenti -117G-hyeA3A-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N57G

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTRE-BI-hGRAD

    Plasmid

    #207837

    Purpose

    Inducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    mCherry
    hFBXW11 (FBXW11 Human)
    VHH-GFP4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    215 amino acids of the full length protein

    Promoter

    TRE3G BI promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. lentiCRISPR v2-sgSLC7A11/xCT-1

    Plasmid

    #161818

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. lentiCRISPR v2-sgSLC7A11/xCT-2

    Plasmid

    #161819

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    solute carrier family 7 member 11 (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pESF140_HisSpy_DDB1∆B

    Plasmid

    #124213

    Purpose

    Insect cell expression vector for His6, Spy tagged hsDDB1∆B

    Depositor

    Eric Fischer

    Article

    Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

    Insert

    DNA damage-binding protein 1 (DDB1 Human)
    

    Use

    Tags

    His6-Spy

    Expression

    Insect

    Mutation

    internal deletion of BPB domain to aid crystalliz…

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSAM2_mCherry_Gata4

    Plasmid

    #72690

    Purpose

    Doxycyline regulated expression of Gata4 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Gata 4 (Gata4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. H3S10ph biosensor

    Plasmid

    #120809

    Purpose

    FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Threonine 3, Threonine 6, and Threonine 11 are mu…

    Promoter

    CMV

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSAM2_mCherry_SRF

    Plasmid

    #72695

    Purpose

    Doxycyline regulated expression of SRF in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    serum response factor (SRF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSAM2_mCherry_Isl1

    Plasmid

    #72696

    Purpose

    Doxycyline regulated expression of Isl1 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Islet1 (Isl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge