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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,821 results for: 158

Showing: 6781 - 6810 of 9821 results

  2. pSCL.113

    Plasmid
    #184982
    Purpose
    Test effect of extending a1/a2 on FAA1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
    Use
    Tags
    Expression
    Yeast
    Mutation
    FAA1 donor P233stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184982-plasmid-map-sequence-id-376532

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  5. pSCL.039

    Plasmid
    #184973
    Purpose
    Test effect of extending a1/a2 on ADE2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
    Use
    Tags
    Expression
    Yeast
    Mutation
    ADE2 donor P272stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184973-plasmid-map-sequence-id-376529

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  6. nPPARg429-gs10-NLS-VPR

    Plasmid
    #188266
    Purpose
    Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    nPPARg429 (PPARG Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188266-plasmid-map-sequence-id-374531

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  9. pYL237

    Plasmid
    #173194
    Purpose
    CRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene
    Depositor
    Thomas Cech
    Article
    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:
    Insert
    EZH2 RNA-binding mutant
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    173194-plasmid-map-sequence-id-360541

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  11. pV5endoNA2

    Plasmid
    #174729
    Purpose
    Expresses V5-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.
    Depositor
    Jukka Finne
    Article
    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-
    Insert
    V5 epitope tag
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174729-plasmid-map-sequence-id-343520

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  20. pSTAP-seq_fly-4xUAS

    Plasmid
    #125149
    Purpose
    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells
    Depositor
    Alexander Stark
    Article
    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:
    Insert
    4 x upstream activating sequence (UAS)
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    125149-plasmid-map-sequence-id-247994

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Showing: 6781 - 6810 of 9821 results