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pET-21a(+)-DR RecR

Plasmid

#12617

Depositor

SeWon Suh

Publication

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR (recR Deinococcus radiodurans)

Tags

His

Expression

Bacterial

Available since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pU6_rd12_nsgRNA(PP7)

Plasmid

#232436

Purpose

nsgRNA for PE3b correction of the rd12 mutation, contains a PP7 in the scaffold tetraloop

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PP7-tagged nicking gRNA (PE3b) for rd12 correction driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDN0606 (pDEST-DHFR F[3] N-term,HygR)

Plasmid

#210488

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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NIR-AG

Plasmid

#210728

Purpose

NIR light-activated GSDMD

Depositor

Vladislav Verkhusha

Publication

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NIR-AG (GSDMD Human)

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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DDX5 D248N V5

Plasmid

#199577

Purpose

Expresses helicase dead DDX5 cloned into pLenti puro

Depositor

Michael Kastan

Publication

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

DDX5 (DDX5 Human)

Tags

V5

Expression

Mammalian

Mutation

D248N

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLV U6-sgRNA UbC-GFP

Plasmid

#106948

Purpose

sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

Depositor

Albert Edge

Publication

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

sgRNA BsmBI/Esp3I cloning site

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-DHX36iso2E335A

Plasmid

#159588

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cells

Depositor

Markus Hafner

Publication

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

E335A

Promoter

CMV

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgWRN1

Plasmid

#125771

Purpose

constitutive expression of a guide RNA targeting human WRN

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgWRN1 (WRN Human)

Use

CRISPR

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgWRN2

Plasmid

#125772

Purpose

constitutive expression of a guide RNA targeting human WRN

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgWRN2 (WRN Human)

Use

CRISPR

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSL6 - Brr2 del247 WT

Plasmid

#90256

Purpose

protein expression

Depositor

Christine Guthrie

Publication

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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Munc18cwt-pDEST27

Plasmid

#85626

Purpose

GST tagged Munc18c

Depositor

David James

Publication

Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

Insert

Munc18c (Stxbp3 Mouse)

Tags

GST

Expression

Mammalian

Promoter

CMV

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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JW617

Plasmid

#64809

Purpose

CUC3 expression in yeast

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC3 (CUC3 Mustard Weed)

Tags

GAL4 DNA binding domain and Myc

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pet23a_RBPMS_FL

Plasmid

#59392

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Publication

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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mTFP1-CytERM-N-17

Plasmid

#55476

Purpose

Localization: Endoplasmic Reticulum, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Publication

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

CytERM

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
p413TEF-PYK2

Plasmid

#34606

Depositor

Markus Ralser

Publication

Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

Insert

PYK2 (PYK2 Budding Yeast)

Expression

Yeast

Promoter

TEF

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pU6_rd12_pegRNA_(PP7-C4-Q1)

Plasmid

#232435

Purpose

pegRNA with optimized 3' modifications to correct the rd12 mutation

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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PAC_E83_LOV_eGFP

Plasmid

#231574

Purpose

This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

Depositor

Dominik Niopek

Publication

Wolf et al Nat Methods. 2025 Aug;22(8):1698-1706. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83

Expression

Mammalian

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pInducer20 TIFA T9A

Plasmid

#231590

Purpose

TIFA gene mutated in position T9A (mutation on phosphorylation site) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Publication

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

TIFA (TIFA Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

T9A

Promoter

doxycycline

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2

Plasmid

#231372

Purpose

Express human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Publication

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Synthetic, Human)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry-C1-ORP5deltaORDdeltaTMD-FKBP

Plasmid

#220074

Purpose

expresses ORP5 with the OSBP-related ligand-binding domain and the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

Depositor

Gerry Hammond

Publication

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

OSBPL5deltaORDdeltaTMD (OSBPL5 Human)

Expression

Mammalian

Mutation

deltaORDdeltaTMD

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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