We narrowed to 4,997 results for: mcs

1. pNP1830

   Plasmid

   #222728

   Purpose

   pNP561 - T5 Target with T7 Inducible IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Recombinase
   

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNP2157

   Plasmid

   #222732

   Purpose

   pNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Helper (WT)
   

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNP1047

   Plasmid

   #222726

   Purpose

   pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 bridge RNA
   

   Tags

   sfGFP

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMP2463

   Plasmid

   #107774

   Purpose

   Expression of EGFP

   Depositor

   Herman Spaink

   Article

   Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   pLacZ-EGFP in pBBR1-mcs5
   

   Tags

   pLacZ-EGFP

   Expression

   Bacterial

   Promoter

   pLacZ

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMP4518

   Plasmid

   #107778

   Purpose

   Expression of EYFP

   Depositor

   Herman Spaink

   Article

   Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   pLacZ-EYFP in pBBR1-mcs5
   

   Tags

   pLacZ-EYFP

   Expression

   Bacterial

   Promoter

   pLacZ

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNP555

   Plasmid

   #222722

   Purpose

   sfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Type

   Empty backbone

   Tags

   sfGFP

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMP4516

   Plasmid

   #107775

   Purpose

   Expression of ECFP

   Depositor

   Herman Spaink

   Article

   Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   pLacZ-ECFP in pBBR1-mcs5
   

   Tags

   pLacZ-ECFP

   Expression

   Bacterial

   Promoter

   pLacZ

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBR5pemIK-GW

   Plasmid

   #126556

   Purpose

   Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatible

   Depositor

   Lindsey Burbank, Drake Stenger

   Article

   Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNP2270

   Plasmid

   #222733

   Purpose

   pNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Helper (lacZ)
   

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHL 651

    Plasmid

    #53016

    Purpose

    LacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Inserts

    LacIq
    tetR
    

    Use

    Synthetic Biology

    Tags

    RBS(st3) and T1 terminator

    Expression

    Bacterial

    Promoter

    pCon

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

11. JM3 [JBEI-6891]

    Plasmid

    #89788

    Purpose

    Broad host range vector pBBR1-MCS2 containing 'tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli) under control of BAD promoter

    Depositor

    Harry Beller

    Article

    Muller et al Appl Environ Microbiol. 2013 Jul;79(14):

    Insert

    tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli)
    

    Expression

    Bacterial

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBiFC-HA-hCaspase 2 FL (C303S)-VC155

    Plasmid

    #49257

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 (CASP2 Human)
    

    Tags

    HA and split Venus C

    Expression

    Mammalian

    Mutation

    C303S

    Promoter

    CMV

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pBiFC-HA-hCaspase 2 prodomain-VC155

    Plasmid

    #49261

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 prodomain (CASP2 Human)
    

    Tags

    HA and split Venus C

    Expression

    Mammalian

    Mutation

    delete aa 148-435

    Promoter

    CMV

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pBIFC-flag-hCaspase 2 prodomain-VN173

    Plasmid

    #49262

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 prodomain (CASP2 Human)
    

    Tags

    flag and split Venus N

    Expression

    Mammalian

    Mutation

    delete aa 148-435

    Promoter

    CMV

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pBiFC-HA-hCaspase 2 CARD-VC155

    Plasmid

    #49259

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    Caspase-2 CARD (CASP2 Human)
    

    Tags

    HA and split Venus C

    Expression

    Mammalian

    Mutation

    deleted aa 123-435

    Promoter

    CMV

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pBiFC-flag-hCaspase 2 FL (C303S)-VN173

    Plasmid

    #49258

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 (CASP2 Human)
    

    Tags

    flag and split Venus N

    Expression

    Mammalian

    Mutation

    C303S

    Promoter

    CMV

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pBiFC-flag-hCaspase 2 CARD-VN173

    Plasmid

    #49260

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 CARD (CASP2 Human)
    

    Tags

    Flag and split Venus N

    Expression

    Mammalian

    Mutation

    delete aa 123-435

    Promoter

    CMV

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

18. T7pr-His6-MBP-TEV-dCas9-BirA*

    Plasmid

    #159994

    Purpose

    Bacterial expression of dCas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    dCas9-BirA*
    

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. T7pr-His6-MBP-TEV-Cas9-BirA*

    Plasmid

    #159993

    Purpose

    Bacterial expression of Cas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    Cas9-BirA*
    

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTetOFF SNCA-A53T

    Plasmid

    #215372

    Purpose

    All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    SNCA (SNCA Human)
    

    Expression

    Mammalian

    Mutation

    Ala53Thr

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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