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pEN243-EN1953

Plasmid

#224546

Purpose

Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl (Nipbl Mouse)

Expression

Mammalian

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEN243-KH217

Plasmid

#224550

Purpose

Cas9-2A-puro and sgRNA targeting the STOP codon mouse Car2

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

Cas9 and sgRNA targeting the STOP codon of mouse CAR2 (Car2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-Gal9NFcLALAPGhole6xHis

Plasmid

#237381

Purpose

pTwist CMV BetaGlobin encoding Galectin-9 N terminal carbohydrate binding domain-Fc fusion with LALA P329G hole Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

Galectin-9-silentFc (N-terminal carbohydrate binding domain only) (LGALS9 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-PDE6B-146

Plasmid

#239098

Purpose

pGL3 luciferase vector with 146bp PDE6B promoter (containing NRL response element).

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Insert

PDE6B promoter (PDE6B Human)

Use

Luciferase

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-U6-sgRXRa4-1; EF1a-mScarlet

Plasmid

#242889

Purpose

For lentiviral Crispr/Cas9 mediated knockout of mouse RXRa, using a guide RNA against exon 4, coupled to EF1a-driven mScarlet

Depositor

Elaine Fuchs

Article

Stewart et al Nature. 2024 Sep;633(8029):407-416. doi:

Insert

sgRNA targeting Rxra

Use

CRISPR and Lentiviral

Tags

mScarlet

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBD-PYR1

Plasmid

#241278

Purpose

Yeast two hybrid vector expressing BD-PYR1 (wild type)

Depositor

Sean Cutler

Article

Park et al Science. 2009 May 22;324(5930):1068-71.

Insert

PYR1 (PYR1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 PDZD8-CT

Plasmid

#227854

Purpose

To express in E. coli PDZD8-CT

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

PDZD8 (PDZD8 Human)

Tags

GST

Expression

Bacterial

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139A

Plasmid

#223560

Purpose

Alias: Gαi bONE-GO W139A. Lentiviral vector expressing GINIP-Nluc W139A after a low efficiency IRES downstream of YFP-CAAX placed after the promotor

Depositor

Mikel Garcia-Marcos

Article

Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

Insert

YFP(Venus) - KRas4b - IRES* - human GINIP W139A - linker(GGGS) - Nluc

Use

Lentiviral

Expression

Mammalian

Mutation

W139A mutation in GINIP sequence

Promoter

CMV

Available Since

March 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-Tet-Off-FLEX-DEST (JDW 1186)

Plasmid

#229820

Purpose

An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

AAV

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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HR700_HA2TP53_Ex5

Plasmid

#228860

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 5

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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HR700_HA2TP53_Ex6

Plasmid

#228861

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 6

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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HR700_HA2TP53_Ex7

Plasmid

#228862

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 7

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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HR700_HA2TP53_Ex8

Plasmid

#228863

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 8

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Str-KDEL_SBP-EGFP-CD59

Plasmid

#222307

Purpose

Synchronize the trafficking of CD59 from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and CD59 fused to SBP-EGFP (CD59 Human)

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAMP1-Y414A

Plasmid

#222319

Purpose

Synchronize the trafficking of LAMP1-Y414A from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1-Y414A fused to SBP-EGFP (LAMP1 Human)

Expression

Mammalian

Mutation

Tyr414 to Ala

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-LAMP1-Y414A

Plasmid

#222320

Purpose

Synchronize the trafficking of LAMP1-A17 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1-Y414A fused to SBP-mCherry (LAMP1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CTLA-4-GCN4-His6

Plasmid

#223582

Purpose

Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CTLA4 (CTLA4 Human)

Tags

GCN4, His6 and Twin-Strep, SNAP

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CD80 (I92R)-His10

Plasmid

#223580

Purpose

Protein purification of human CD80 (I92R) with Twin-Strep, SNAP and 10xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Tags

His10 and Twin-Strep, SNAP

Expression

Mammalian

Mutation

I92R

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CD80-His10

Plasmid

#223579

Purpose

Protein purification of human CD80 with Twin-Strep, SNAP and 10xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Tags

His10 and Twin-Strep, SNAP

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ILF3-ts1

Plasmid

#174265

Purpose

ILF3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ILF3 shRNA (ILF3 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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