We narrowed to 17,203 results for: puro

1. mito-EcSTH

   Plasmid

   #211921

   Purpose

   Mitochondrial localization of EcSTH

   Depositor

   Binghui Li

   Article

   Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

   Insert

   mito-EcSTH
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLVX-PERK(LD)-EGFP-HOTag3

   Plasmid

   #225678

   Purpose

   Used to generate stable cell lines that express PERK(LD)-EGFP-HOTag3 and to detect the unfolded proteins in the ER lumen.

   Depositor

   Likun Wang

   Article

   Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PLKO.1-Scrambled

   Plasmid

   #136035

   Purpose

   Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   None (Scrambled)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LentiCRISPRv2-Opti

   Plasmid

   #163126

   Purpose

   LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti UBC LysoFlux (HaloTag7-mGFP-Galectin3)

   Plasmid

   #215008

   Purpose

   Express a Lysophagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLVX-PERK(LD)-EGFP

   Plasmid

   #225680

   Purpose

   Used to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

   Depositor

   Likun Wang

   Article

   Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPB-cT3G-cERP2-NELFE

   Plasmid

   #222559

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of NELFE

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   NELFE (NELFE Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO-Tet-On-ARNTL-shRNA2

   Plasmid

   #166914

   Purpose

   Lentivirus for inducible knockdown of human ARNTL(BMAL1)

   Depositor

   Roland Friedel

   Insert

   ARNTL shRNA (BMAL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAV-U6+27-Tornado-BroccoliSamSensor#1

   Plasmid

   #124363

   Purpose

   Overexpression of circular RNA (Broccoli-based SAM sensor)

   Depositor

   Samie Jaffrey

   Article

   Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

   Insert

   Tornado-BroccoliSamSensor#1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TetO::EGFP-hMeikin

    Plasmid

    #174705

    Purpose

    Doxycycline inducible expression of human Meikin

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO-FOXM1-shRNA-81

    Plasmid

    #89495

    Purpose

    Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.

    Depositor

    Adam Karpf

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFLAG/HA-TNRC6B

    Plasmid

    #10979

    Depositor

    Thomas Tuschl

    Article

    Meister et al Curr Biol. 2005 Nov 9. ():.

    Insert

    TNRC6B (TNRC6B Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p663-UBC-miniTurbo-V5-PKN3_IDG-K

    Plasmid

    #170601

    Purpose

    Gateway destination clone of PKN3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-PKN3 (PKN3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV.MRE11i

    Plasmid

    #15565

    Depositor

    Didier Trono

    Article

    Ariumi et al J Virol. 2006 Mar . 80(5):2445-52.

    Insert

    MRE11 shRNA (MRE11 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

15. PresentER-Cassette GFP

    Plasmid

    #102942

    Purpose

    PresentER with removable cassette for cloning of MHC-I antigen (GFP version)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQCXIP-GFP-LacR

    Plasmid

    #59418

    Purpose

    retroviral construct expressing the Lac repressor (LacR) tagged with GFP

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Lac repressor (LacR)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pDest-667 (attR4-attR3)

    Plasmid

    #161886

    Purpose

    Multisite Gateway (attR4-attR3) vector for lentivirus production

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCV-Peredox-mCherry-NLS

    Plasmid

    #32385

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCherry-NLS
    

    Use

    Retroviral

    Tags

    Nuclear Localization Seq

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PresentER-Cassette mCherry

    Plasmid

    #102943

    Purpose

    PresentER with removable cassette for cloning of MHC-I antigen (mCherry version)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDest-663 (attR4-attR2)

    Plasmid

    #161884

    Purpose

    Multisite Gateway (attR4-attR2) vector for lentivirus production

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LPCX Smad3

    Plasmid

    #12638

    Depositor

    Rik Derynck

    Article

    Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2006

    Availability

    Academic Institutions and Nonprofits only

22. pCAG-HA-Sox2-IP

    Plasmid

    #13459

    Depositor

    Shinya Yamanaka

    Article

    Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLKO-Tet-On-ARNTL-shRNA1

    Plasmid

    #166913

    Purpose

    Lentivirus for inducible knockdown of human ARNTL(BMAL1)

    Depositor

    Roland Friedel

    Insert

    ARNTL shRNA (BMAL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p663-UBC-2xV5-TurboID-NES-STOP_IDG-K

    Plasmid

    #158162

    Purpose

    Gateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    2xV5-TurboID-NES
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLPCX-IRES-GFP

    Plasmid

    #65436

    Purpose

    For transfection or retroviral production. Mammalian expression of EGFP driven by the ECMV IRES.

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    IRES-GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLPC-NMYC TRF2 deltaB

    Plasmid

    #16067

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF2 (TERF2 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Deleted Basic Domain (aa1-44)

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

27. pLPC-NMYC TRF2 deltaM

    Plasmid

    #16068

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF2 (TERF2 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Deleted Myb Domain (aa454-500)

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pLKO-FOXM1-shRNA-84

    Plasmid

    #89494

    Purpose

    Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.

    Depositor

    Adam Karpf

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LV-TRE-Empty-T2A-dsRedExpress2

    Plasmid

    #60623

    Purpose

    Expresses dsRedExpress2 in response to doxycycline

    Depositor

    Charles Gersbach

    Article

    Kabadi et al ACS Synth Biol. 2014 Dec 10.

    Insert

    Empty
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p663-UBC-V5-3xHA-miniTurbo-3XNLS-STOP_IDG-K

    Plasmid

    #158160

    Purpose

    Gateway destination clone of miniTurbo-3XNLS (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    V5-3xHA-miniTurbo-3XNLS
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAGTetRnls

    Plasmid

    #26599

    Depositor

    Peter Andrews

    Article

    Zafarana et al Stem Cells. 2009 Apr . 27(4):776-82.

    Insert

    Tetracycline repressor (TetR E.Coli)
    

    Use

    Tags

    nls

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLKO-Tet-On-ARNTL-shRNA3

    Plasmid

    #166915

    Purpose

    Lentivirus for inducible knockdown of human ARNTL(BMAL1)

    Depositor

    Roland Friedel

    Insert

    ARNTL shRNA (BMAL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPB-cT3G-cERP2-SYMPK

    Plasmid

    #222556

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of SYMPK

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    SYMPK (SYMPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LentiGuide-BC-start

    Plasmid

    #127168

    Purpose

    Entry plasmid for two-step guide-barcode cloning

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    N/A

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLKO.1C-shSCR

    Plasmid

    #139472

    Purpose

    Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

    Depositor

    David Cobrinik

    Article

    Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

    Insert

    shSCR
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMA3160

    Plasmid

    #35043

    Depositor

    Mikhail Alexeyev

    Article

    Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLPC-C-SF-TAP-DEST-TLK1-WT

    Plasmid

    #218829

    Purpose

    Expresses C-terminal Strep-FLAG-tagged wild type human TLK1 variant 5 in a retrovirus vector.

    Depositor

    Travis Stracker

    Insert

    TLK1 (TLK1 Human)
    

    Use

    Tags

    FLAG-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCI 9.0

    Plasmid

    #74229

    Purpose

    Luminescent coincidence reporter with FLAG tags, FLuc-P2A-NLucPEST

    Depositor

    James Inglese

    Article

    Schuck et al Curr Protoc Neurosci. 2017 Apr 10;79:5.3

    Insert

    3xFlag-FLuc-P2A-3xFlag-NLucPEST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    None

    Available since

    May 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTripz Sox4.965

    Plasmid

    #101120

    Purpose

    shRNA Vector

    Depositor

    Joan Massague

    Article

    David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

    Insert

    Sox4.965
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. psd44-G13WT

    Plasmid

    #46829

    Purpose

    expression of wild type G protein alpha 13 in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    GNA13 (GNA13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK promoter

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only