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FRET-ON

Plasmid

#254591

Purpose

Engineered high-FRET standard for FRET ratio calibration

Depositor

Chuan-Hsiang Huang

Publication

Wu et al iScience. 2025 Oct 11;28(11):113743. doi

Insert

FRET-ON

Use

Lentiviral

Tags

ECFP and YPet

Expression

Mammalian

Mutation

Y349F

Promoter

CMV

Available since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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FRET-OFF

Plasmid

#254590

Purpose

Engineered low-FRET standard for FRET ratio calibration

Depositor

Chuan-Hsiang Huang

Publication

Wu et al iScience. 2025 Oct 11;28(11):113743. doi

Insert

FRET-OFF

Use

Lentiviral

Tags

ECFP and YPet

Expression

Mammalian

Mutation

deleted Histone H3 from H3K9me3(W45A) biosensor

Promoter

CMV

Available since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-YPet

Plasmid

#244039

Purpose

anti mTFP1DARPin_G01 fused to YPet

Depositor

Markus Affolter

Publication

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-YPet

Expression

Mammalian

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-YPet-H2B

Plasmid

#244034

Purpose

anchoring anti mTFP1DARPin_E11 fused to YPet to chromatin/nucleus

Depositor

Markus Affolter

Publication

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-YPet-HIST1H2BJ

Expression

Mammalian

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-YPet-CAAX

Plasmid

#244033

Purpose

anchoring anti mTFP1DARPin_G01 fused to YPet to plasma membrane

Depositor

Markus Affolter

Publication

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-YPet-CAAX

Tags

CAAX

Expression

Mammalian

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-YPet-CAAX

Plasmid

#244032

Purpose

anchoring anti mTFP1DARPin_E11 fused to YPet to plasma membrane

Depositor

Markus Affolter

Publication

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-YPet-CAAX

Tags

CAAX

Expression

Mammalian

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-YPet

Plasmid

#244038

Purpose

anti mTFP1DARPin_E11 fused to YPet

Depositor

Markus Affolter

Publication

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-YPet

Expression

Mammalian

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-mCherry

Plasmid

#244029

Purpose

anti mTFP1DARPin_G01 fused to mCherry

Depositor

Markus Affolter

Publication

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-mCherry

Expression

Mammalian

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-YPet-H2B

Plasmid

#244035

Purpose

anchoring anti mTFP1DARPin_G01 fused to YPet to chromatin/nucleus

Depositor

Markus Affolter

Publication

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-YPet-HIST1H2BJ

Expression

Mammalian

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-mCherry

Plasmid

#244028

Purpose

anti mTFP1DARPin_E11 fused to mCherry

Depositor

Markus Affolter

Publication

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-mCherry

Expression

Mammalian

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mTFP1-Rab5c

Plasmid

#244031

Purpose

fusion of Rab5c to mTFP1

Depositor

Markus Affolter

Publication

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

mTFP1-Rab5c

Expression

Mammalian

Available since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGA2-tail-YPet (GFFKR/GAAKR)

Plasmid

#145144

Purpose

Shuttle vector with YPet-tagged ITGA2 cytoplasmic tail as an insert with a GFFKR/GAAKR mutation

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGA2-tail-YPet (GFFKR/GAAKR) (ITGA2 Human)

Use

Gateway Cloning

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pNHA9

Plasmid

#223642

Purpose

pUC18 plasmid with rpsM promoter

Depositor

François-Xavier Campbell-Valois

Publication

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA13

Plasmid

#223645

Purpose

Expression of mxiE-turboID fusion

Depositor

François-Xavier Campbell-Valois

Publication

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

mxiE (mxiE )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA14

Plasmid

#223646

Purpose

Expression of turboID-mxiE fusion

Depositor

François-Xavier Campbell-Valois

Publication

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

mxiE (mxiE )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAMC070n1a12

Plasmid

#209746

Purpose

ColA BBa_B0014 tetAp SD8 ScTrpRS rrnB1 proB SD8 Lum1PylRS BBaB10014RNAItrpA+ J23100 SD8 MmPylRS T3Te-T7Te DHFR

Depositor

Ahmed Badran

Publication

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

tRNA operon - ScTrpRS - Lum1PylRS - MmPylRS

Expression

Bacterial

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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2COX8-BmTyr

Plasmid

#222943

Purpose

mitochondrion-localized BmTyr

Depositor

Itaru Hamachi

Publication

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

2COX8-BmTyr-Flag

Tags

Flag tag

Expression

Mammalian

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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IgK-BmTyr-KDEL

Plasmid

#222942

Purpose

ER-localized BmTyr

Depositor

Itaru Hamachi

Publication

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

IgK-BmTyr-Flag-KDEL

Tags

Flag tag

Expression

Mammalian

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-BmTyr-H2B

Plasmid

#222941

Purpose

H2B-BmTyr fusion

Depositor

Itaru Hamachi

Publication

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

BmTyr-H2B

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pNoT7

Plasmid

#218168

Purpose

pET31b plasmid with short insert lacking T7 and ribosome binding sites as control

Depositor

Emily Weinert

Publication

Chauhan et al ACS Cent Sci. 2022 Nov 23;8(11):1518-152

Insert

Short sequence, mutated RBS and T7 sites

Expression

Bacterial

Promoter

NA - mutated T7

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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