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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11,122 results for: ARIA;

Showing: 4561 - 4580 of 11122 results

  1. SnFR_V.7.1.2

    Plasmid
    #182809
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Insert
    V.7.1.2-CholineSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    182809-plasmid-map-sequence-id-358174

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  5. pLS1/-11

    Plasmid
    #31492
    Depositor
    Kathleen Matthews
    Insert
    Lactose repressor with 11 amino acid C-terminal deletion (lacI E. coli variant)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 21, 2012
    Availability
    Academic Institutions and Nonprofits only

  6. KP.3

    Plasmid
    #233340
    Purpose
    expressing SARS-CoV-2 KP.3 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (KP.3) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    F456L, Q493E, V1104L on the basis of JN.1
    Promoter
    CMV
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    233340-plasmid-map-sequence-id-472420

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  7. pJK648

    Plasmid
    #71611
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp mutant (TdPurE2-F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes phenylalanine-79 to tryptophan
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71611-plasmid-map-sequence-id-131688

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  8. XEC

    Plasmid
    #233341
    Purpose
    expressing SARS-CoV-2 XEC spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XEC) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T22N, F59S on the basis of KP.3
    Promoter
    CMV
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    233341-plasmid-map-sequence-id-472417

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  9. KP.3.1.1

    Plasmid
    #233342
    Purpose
    expressing SARS-CoV-2 KP.3.1.1 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (KP.3.1.1) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S31del on the basis of KP.3
    Promoter
    CMV
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    233342-plasmid-map-sequence-id-472416

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  10. NOVA1 eGFP

    Plasmid
    #61275
    Purpose
    GFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    NOVA1 (NOVA1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61275-plasmid-map-sequence-id-99586

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  11. pcDNA3.3_SARS2_XBB.1.5

    Plasmid
    #196585
    Purpose
    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XBB.1.5)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G252V, F486P on the basis of XBB
    Promoter
    CMV
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196585-plasmid-map-sequence-id-386779

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  12. pCas9CR5-NG

    Plasmid
    #120228
    Purpose
    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120228-plasmid-map-sequence-id-233263

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  13. pcDNA3.3_SARS2_EG.5.1

    Plasmid
    #207641
    Purpose
    pseudovirus plasmid that expresses spike of EG.5.1
    Depositor
    David Nemazee
    Insert
    Spike (EG.5.1) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Q52H, F456L on the basis of XBB.1.5
    Promoter
    CMV
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    207641-plasmid-map-sequence-id-437731

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  14. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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  16. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid
    #195487
    Purpose
    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    C61T, C262V
    Promoter
    T7
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195487-plasmid-map-sequence-id-393190

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  19. pCas9CR5

    Plasmid
    #120227
    Purpose
    pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
    Depositor
    Christopher Reisch
    Insert
    SpCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Stop codon inserted before ssrA degradation tag
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120227-plasmid-map-sequence-id-233288

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  20. pJK505

    Plasmid
    #71594
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, additiona…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71594-plasmid-map-sequence-id-131669

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Showing: 4561 - 4580 of 11122 results