We narrowed to 13,337 results for: Mpl;

1. CTAG_NLS-SV40

   Plasmid

   #136108

   Purpose

   CTAG Nuclear localiation signal SV40 for fusion with CDS12

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CTAG_NLS-SV40
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCsB_spacer

   Plasmid

   #136076

   Purpose

   Use when making odd level constructs (L3,...) if pCsB position (position 2) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Even spacer
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. CDS12_mTurquoise2-linker

   Plasmid

   #136090

   Purpose

   FP for N-term fusion with a CTAG without linker

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS12_mTurquoise2(noStop)-SerGly10xAlaSer
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. CDS12_mVenus-linker

   Plasmid

   #136092

   Purpose

   FP for N-term fusion with a CTAG without linker

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS12_mVenus(noStop)-SerGly10xAlaSer
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. CDS12_TP-MpSIG2

   Plasmid

   #136093

   Purpose

   Chloroplast transit peptide from MpSIG2. For N-term fusion with a CTAG

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   TP-MpSIG2, Mapoly0214s0004
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCsA_spacer

   Plasmid

   #136075

   Purpose

   Use when making odd level constructs (L3,...) if pCsA position (position 1) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Even spacer
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pIVT-EGFP-ratCENP-C

   Plasmid

   #174445

   Purpose

   in vitro transcription template for rat CENP-C (N-ter EGFP tagged)

   Depositor

   Michael Lampson

   Article

   Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

   Insert

   Centromere protein C
   

   Use

   In vitro transcription template

   Tags

   EGFP

   Promoter

   T7 SP6

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIVT-EGFP-pumilioCENP-C

   Plasmid

   #174446

   Purpose

   in vitro transcription template for R. pumilio CENP-C (N-ter EGFP tagged)

   Depositor

   Michael Lampson

   Article

   Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

   Insert

   Centromere protein C
   

   Use

   In vitro transcription template

   Tags

   EGFP

   Promoter

   T7 SP6

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJH858

   Plasmid

   #172176

   Purpose

   PylB overexpression and purification

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   PT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]
   

   Expression

   Bacterial

   Mutation

   pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJH859

    Plasmid

    #172177

    Purpose

    PylB overexpression and purification

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
    

    Expression

    Bacterial

    Mutation

    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJH580

    Plasmid

    #172164

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (pylBCD 3f2v2+B); AraC
    

    Expression

    Bacterial

    Mutation

    PylB(N61S, E84K, E122K, E178K, G188R, E251D, G332…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJH750

    Plasmid

    #172170

    Purpose

    Microscopy inclusion body assay

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    pCDF.3f2, deleted sumo
    

    Expression

    Bacterial

    Mutation

    Evolved PylBCD operon; SUMO tag deleted from PylB…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJH751

    Plasmid

    #172171

    Purpose

    Microscopy inclusion body assay

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    pCDF.JM10.1, deleted sumo
    

    Expression

    Bacterial

    Mutation

    Evolved PylBCD operon; SUMO tag deleted from PylB…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJH768v2

    Plasmid

    #172173

    Purpose

    PylB overexpression and purification

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    PT7/Lac-[His6-TEV-SUMO-PylB.3f2]
    

    Expression

    Bacterial

    Mutation

    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJH769v2

    Plasmid

    #172174

    Purpose

    PylB overexpression and purification

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]
    

    Expression

    Bacterial

    Mutation

    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJH191

    Plasmid

    #172149

    Purpose

    Accessory Plasmid for PACE of pylBCD variants

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Ppsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
    

    Expression

    Bacterial

    Mutation

    gIII(P29*, Y184*); [strong promoter eG, strong RB…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJH61

    Plasmid

    #172143

    Purpose

    Accessory Plasmid for PACE of pylBCD variants

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] pylS; PproK pylT
    

    Expression

    Bacterial

    Mutation

    gIII(P29*); [strong promoter eG, strong RBS SD8] …

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJH190

    Plasmid

    #172148

    Purpose

    Accessory Plasmid for PACE of pylBCD variants

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
    

    Expression

    Bacterial

    Mutation

    gIII(P29*); [strong promoter eG, strong RBS SD8] …

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTX1-SMN2-exon7-ESE1

    Plasmid

    #126042

    Purpose

    In-vitro-transcription of SMN2-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

    Depositor

    Frédéric Allain

    Article

    Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

    Insert

    SMN2 exon7 ESE1
    

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher

    Plasmid

    #157674

    Purpose

    Expression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    SpyCatcher with ddFLN4, ELP, Histag and ybbR tag
    

    Tags

    ddFLN4, ELP, Histag and ybbR tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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