We narrowed to 6,654 results for: SIM;

1. pAAV-COX4-dAPEX2

   Plasmid

   #117176

   Purpose

   Plasmid name in publication: pAAV-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   COX4-dAPEX2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAT9651-BEAR-GFP

   Plasmid

   #162989

   Purpose

   BEAR target plasmid with split EGFP and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAD-LyseR (in lacZ deficient strain)

   Plasmid

   #99245

   Purpose

   For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   phage lambda gene R (R phage lambda)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   beta-lactamase promoter

   Available since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR- GAL4-UAS-BAMH1-IRES-mCherry-PGK-BFP

   Plasmid

   #223215

   Purpose

   Lentiviral vector - mCherry reporter with a BAMH1 site for Gal4DBDVP64 synNotch receptors with a constitutive BFP

   Depositor

   Wendell Lim

   Article

   Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

   Insert

   mCherry BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TL1 alphaL_DdCBE 3-4 (left, DddA11-C) - TL1 alphaDdCBE 1 (left, DddA11-C)

   Plasmid

   #221998

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-3/4 left, alpha-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | DddA: T1413I

   Promoter

   CMV

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCyRFP1-RhoA

   Plasmid

   #84358

   Purpose

   fusion protein of RhoA, FRET donor

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mCyRFP1-RhoA (RHOA Synthetic, Human)
   

   Use

   Tags

   mCyRFP1 N terminal fusion to RhoA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScalps_puro_mIL-2Rα

   Plasmid

   #59917

   Purpose

   Expresses mouse interleukin-2 receptor alpha chain

   Depositor

   Silvia Monticelli

   Article

   Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

   Insert

   interleukin 2 receptor alpha (Il2ra Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pLX-B3omega1 (GB1694)

   Plasmid

   #160643

   Purpose

   pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)

   Depositor

   Juan Antonio García, Fabio Pasin

   Article

   Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSR651

   Plasmid

   #65213

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-1 cap
   

   Use

   AAV

   Tags

   Expression

   Insect

   Mutation

   Promoter

   p10 and polyhedrin

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-C2_SEPT9 i1

    Plasmid

    #71609

    Purpose

    mammalian expression of human SEPT9 i1 with a GFP fusion.

    Depositor

    Elias Spiliotis, Cristina Montagna

    Article

    Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAT9752-BEAR-mScarlet

    Plasmid

    #162991

    Purpose

    BEAR target plasmid with split mScarlet and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    mScarlet split with an intron between amino acids 110-111
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLX-B2 (GB3374)

    Plasmid

    #160636

    Purpose

    pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)

    Depositor

    Juan Antonio García, Fabio Pasin

    Article

    Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. APLF-c001

    Plasmid

    #173077

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    APLF (APLF Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K360-V448, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMJ923

    Plasmid

    #78313

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Use

    Tags

    HA-2xNLS-mCherry-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2TAL3-RR

    Plasmid

    #37276

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Use

    Tags

    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJS288

    Plasmid

    #200714

    Purpose

    To express yeast Hxt1 hexose transporter in yeast

    Depositor

    Jens Boch

    Article

    Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

    Inserts

    PMA1 promoter (PMA1 Budding Yeast)
    Hxt1 (HXT1 Budding Yeast)
    ADH2 Ter (ADH2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    domesticated for MoClo cloning (removal of intern…

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-IGK-dAPEX2-KDEL

    Plasmid

    #117182

    Purpose

    Plasmid name in publication: pAAV-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    IGK-dAPEX2-KDEL
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-C2_SEPT9 i4

    Plasmid

    #71617

    Purpose

    mammalian expression of human SEPT9 i4 with a GFP fusion

    Depositor

    Elias Spiliotis, Cristina Montagna

    Article

    Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLX-Z4 (GB3375)

    Plasmid

    #160648

    Purpose

    pLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)

    Depositor

    Juan Antonio García, Fabio Pasin

    Article

    Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ALC1-c005

    Plasmid

    #173092

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    CHD1L (CHD1L Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    L613-K879, codon optimized

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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