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pSB1C3-pT7-sfGFP_RED20

Plasmid

#135173

Purpose

Expresses sfGFP recoded in the RED20 genetic code off the T7 promoter

Depositor

Drew Endy

Article

Calles et al Nucleic Acids Res. 2019 Sep 12. pii: 556

Insert

sfGFP_RED20

Use

Synthetic Biology

Mutation

Recoded ORF of sfGFP to only use one codon per am…

Promoter

taatacgactcactata

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Nxph4-4Q-HA

Plasmid

#128104

Purpose

Express HA tagged Nxph4-4Q

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nxph4 (Nxph4 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2175

Plasmid

#111890

Purpose

High copy number plasmid containing Ple342 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple342 (TUBB3 Human)

Use

Unspecified

Promoter

Ple342 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2176

Plasmid

#111891

Purpose

High copy number plasmid containing Ple343 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple343 (TUBB3 Human)

Use

Unspecified

Promoter

Ple343 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2177

Plasmid

#111892

Purpose

High copy number plasmid containing Ple344 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple344 (TUBB3 Human)

Use

Unspecified

Promoter

Ple344 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-VQR-P2A-Puro

Plasmid

#110848

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMB-PERr

Plasmid

#61203

Purpose

Fluorescent reporter for peroxisomes mCherry-SKL expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

mCherry-SKL

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-PLAr

Plasmid

#61201

Purpose

Fluorescent reporter for plastids MtRubisco1sp-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRubisco1sp-mCherry

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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tdminiSOG-PCNA-19

Plasmid

#57806

Purpose

Localization: Nucleus, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

PCNA (PCNA Human)

Tags

tdminiSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
ReaLiSM A1-Hinge-GPIba+2cys

Plasmid

#59581

Purpose

express VWF A1-GPIba ReaLiSM construct in mammalian cells

Depositor

Timothy Springer

Article

Kim et al Nature. 2010 Aug 19;466(7309):992-5. doi

Insert

VWF A1-linker-GPIba (VWF Human)

Tags

6His

Expression

Mammalian

Promoter

chick beta-actin

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pLenti-EF1alpha-DEST-IRES-Neo

Plasmid

#253766

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to neomycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pAT2732-(PB-4_EF1α-Hygro_2A_rtTA3 [HA-BFP-KRAB-PUFa])

Plasmid

#251107

Purpose

Expression of KRAB-PUFa component of Casilio-i system

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

BFP-KRAB-PUFa

Tags

HA

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pSicoFlipped-CAG-GFP-WPRE-bGHpA

Plasmid

#231018

Purpose

For strong lentivirus-mediated expression of GFP

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

Enhanced GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Vcan-V3-HA

Plasmid

#238076

Purpose

Expresses Vcan V3 in mice heart tissues

Depositor

Yuxuan Guo

Article

Bai et al Cell Prolif. 2025 Jun 3:e70070. doi: 10.

Insert

Vcan (Vcan Mouse)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_BIP-sfGFP-TurboID-KDEL

Plasmid

#240223

Purpose

Gateway entry clone with ER-localized TurboID tagged with superfolder GFP (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

BIP-sfGFP-TurboID-KDEL

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW mTagBFP2-P2A-NeoR

Plasmid

#239568

Purpose

Cytosolic expression of mTagBFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mTagBFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW mEmerald-P2A-NeoR

Plasmid

#239569

Purpose

Cytosolic expression of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-MCP-SRSF3-V5-mCherry

Plasmid

#235090

Purpose

SRSF3 mCherry fusion protein with MCP domain

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-MCP-SRSF3-V5-mCherry (SRSF3 Human)

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-SEC61B-mCherry-FRB

Plasmid

#233349

Purpose

Stable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

SEC61B (SEC61B Human)
mCherry
FRB

Use

Retroviral

Expression

Mammalian

Mutation

T2098L mutant of FKBP-rapamycin binding domain of…

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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iGFP-gamma-actin

Plasmid

#231554

Purpose

Mammalian expression of human gamma actin fused intramolecularly to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

ACTG1 (ACTG1 Human)

Tags

msfGFP (intramolecular)

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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