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ApoE4_47-300_mCh-SspB (pBS1069)

Plasmid

#185292

Purpose

For the mammalian expression of the human protein ApoE4_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE4_47-300

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE2_47-300_mCh-SspB (pBS1068)

Plasmid

#185291

Purpose

For the mammalian expression of the human protein ApoE2_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE2_47-300

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM 3B-His

Plasmid

#172192

Purpose

Coding sequence of human FAM3B pCS2 C terminal 6xHis tag used for protein purification from conditioned medium

Depositor

Edward De Robertis

Publication

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

hFAM 3B (FAM3B Human)

Tags

His

Expression

Mammalian

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_E1770Q-talin1 (1-2541)

Plasmid

#166121

Purpose

For expression of full-length mouse talin-1 carrying mutation E1770Q in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Publication

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

E1770Q-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

E1770Q

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsXRN1_1650-1706_P

Plasmid

#147250

Purpose

Mammalian Expression of HsXRN1_1650-1706

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

HsXRN1_1650-1706 (XRN1 Human)

Expression

Mammalian

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_RXRB_Hormone-recep

Plasmid

#109849

Purpose

Protein expression and purification of RXRB_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RXRB_Hormone-recep (RXRB Human)

Expression

Bacterial

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_AP2M1_Adap-comp-sub

Plasmid

#109992

Purpose

Protein expression and purification of AP2M1_Adap-comp-sub

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

AP2M1_Adap-comp-sub (AP2M1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-HT_ygiF-FL

Plasmid

#127795

Purpose

Expresses full-length ygiF in E. coli cells

Depositor

Michael Hothorn

Publication

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

ygiF (ygiF Escherichia coli)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1958

Plasmid

#82569

Purpose

High copy number plasmid containing PR2/3:Con MiniPromoter insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

PRS2/3:Con

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCMB-TLEr

Plasmid

#61188

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-scrambled-mCherry-CAAX

Plasmid

#228738

Purpose

Membrane-tethered mCherry scrambled shRNA targeting Miro1

Depositor

Cagla Eroglu

Publication

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Miro1 (Rhot1, Rhot1 Mouse, Rat)

Expression

Mammalian

Available since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-shMiro1-mCherry-CAAX

Plasmid

#228741

Purpose

Membrane-tethered mCherry shRNA targeting Miro1

Depositor

Cagla Eroglu

Publication

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Miro1 (Rhot1, Rhot1 Mouse, Rat)

Expression

Mammalian

Available since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_Vimentin-dHaloTag7_T2A_mEGFP

Plasmid

#187075

Purpose

Expression of dHaloTag7 located at the intermediate filaments of mammalian cells

Depositor

Kai Johnsson

Publication

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

Vimentin-dHaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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PX459-sgRNA048_Grb10

Plasmid

#232934

Purpose

Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

Depositor

Danny Reinberg

Publication

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9-T2A-PuroR (Grb10 S. pyogenes)

Expression

Mammalian

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Vrg4-V5-APEX2-URA

Plasmid

#207071

Purpose

Expressing Vrg4-V5-APEX2. Marker for late Golgi/early endosomes.

Depositor

Zhiping Xie

Publication

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Vrg4

Tags

V5-APEX2

Expression

Yeast

Promoter

pVRG4

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST40-CES2A-ΔC

Plasmid

#200554

Purpose

Mammalian expression of secreted mouse CES2A

Depositor

Jonathan Long

Publication

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAO1_G-alpha

Plasmid

#110013

Purpose

Protein expression and purification of GNAO1_G-alpha

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAO1_G-alpha (GNAO1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESR2_Hormone-recep

Plasmid

#109907

Purpose

Protein expression and purification of ESR2_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESR2_Hormone-recep (ESR2 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_AR_Hormone-recep

Plasmid

#109847

Purpose

Protein expression and purification of AR_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

AR_Hormone-recep (AR Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPB CAG Cdk6 R31C-IRES-EGFP

Plasmid

#153946

Purpose

piggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFP

Depositor

Luca Tiberi

Publication

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Cdk6 (Cdk6 Mouse)

Expression

Mammalian

Mutation

R31C

Promoter

CAG

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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