1. p426 103Q GPD

   Plasmid

   #1184

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

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1. MAP4K1 gRNA (BRDN0001148183)

   Plasmid

   #77608

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP4K1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP4K1 (MAP4K1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MAP4K1 gRNA (BRDN0001145765)

   Plasmid

   #77609

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP4K1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP4K1 (MAP4K1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MAP4K1 gRNA (BRDN0001147227)

   Plasmid

   #77610

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP4K1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP4K1 (MAP4K1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ABL2 gRNA (BRDN0001146662)

   Plasmid

   #77180

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ABL2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ABL2 (ABL2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-GFP/Cre

   Plasmid

   #49056

   Purpose

   Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

   Depositor

   Fred Gage

   Article

   Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

   Insert

   Cre
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   EGFP, Myc, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTRIP TRE-BI GFP10-RHOA/RBD-GFP11

   Plasmid

   #182232

   Purpose

   Inducible coexpression of GFP10-RHOA and RBD-GFP11 domain

   Depositor

   Stéphanie Cabantous

   Article

   Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

   Inserts

   ras homolog family member A (RHOA Human)
   rhotekin RHO binding domain (RBD)
   

   Use

   Lentiviral

   Tags

   GFP10 (split-GFP tripartite) and GFP11 (M4)

   Expression

   Mutation

   Promoter

   TRE bidirectional

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pODN-mNG-RHOA

   Plasmid

   #183836

   Purpose

   Repair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   RHOA homology arms with mNeonGreen-linker (RHOA Human)
   

   Use

   CRISPR; Donor template

   Tags

   mNeonGreen-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJet-CMV-IARS1[W435C]-GFP

   Plasmid

   #212323

   Purpose

   EGFP-tagged IARS1 Trp435Cys

   Depositor

   Sabine Fuchs

   Article

   Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

   Insert

   IARS (IARS1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Trp435cys

   Promoter

   CMV

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKLV2-U6gRNA5(RHOA(15))-PGKpuro2ABFP-W

   Plasmid

   #200493

   Purpose

   Lentiviral vector expressing gRNA targeting human RHOA

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   RHOA(15) (RHOA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKLV2-U6gRNA5(RHOA(12))-PGKpuro2ABFP-W

    Plasmid

    #200492

    Purpose

    Lentiviral vector expressing gRNA targeting human RHOA

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    RHOA(12) (RHOA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CL20_mEGFP_ZC3HAV1_ABL2

    Plasmid

    #205957

    Purpose

    Express mEGFP-tagged fusion protein, ZC3HAV1_ABL2 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    ZC3HAV1_ABL2 (ZC3HAV1, ABL2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DOLK gRNA (BRDN0001149252)

    Plasmid

    #77158

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DOLK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DOLK (DOLK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DOLK gRNA (BRDN0001148537)

    Plasmid

    #77159

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DOLK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DOLK (DOLK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DOLK gRNA (BRDN0001148629)

    Plasmid

    #77160

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DOLK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DOLK (DOLK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DOLK gRNA (BRDN0001149356)

    Plasmid

    #77161

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DOLK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DOLK (DOLK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR223_RHOA_p.Y42C

    Plasmid

    #81306

    Purpose

    Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RHOA (RHOA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Y42C

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CL20_mEGFP_PAX5_NOL4L

    Plasmid

    #205886

    Purpose

    Express mEGFP-tagged fusion protein, PAX5_NOL4L from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    PAX5_NOL4L (PAX5, C20orf112 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE-RHOA-Y42C

    Plasmid

    #116673

    Purpose

    Lentiviral expression of RHOA Y42C

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RHOA (RHOA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Y42C

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223_RHOA_p.Q63L

    Plasmid

    #82181

    Purpose

    Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RHOA (RHOA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Q63L

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223_RHOA_WT

    Plasmid

    #81739

    Purpose

    Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RHOA (RHOA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge