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Plasmid#159671PurposePlasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertExuL-NLS-hSpCas9-T2A-GFP
UseTagsT2A-GFPExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159673PurposePlasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertRcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
UseTagsT2A-GFPExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH
Plasmid#179586PurposeePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)DepositorInsertNLS-NG-T2A-hSHH
UsePiggybacTagsNeonGreenExpressionMutationPromoterTREAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-MCS:
Plasmid#179585PurposeePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette (Red module-dsRed/Green module-NeonGreen)DepositorInsertNLS-NG-T2A-hSHH
UsePiggybacTagsNLS-NG-T2AExpressionMutationPromoterCAGpAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHIE323-LPIV Puro-P2A-miRFP720 ZF6x12-C-DsRed-Express2(x6) phEF1α-BlastR
Plasmid#201539PurposeLanding pad integration vector used to generate ZF6 synTF reporter line (HIE156), assembled with pPD1178 (destination vector), pHIE324-329 (TUPV1-6), pHIE280 (TUPV7), pPD1157 (linker)DepositorInsertsPuromycinR-P2A-miRFP720
ZF6x12-C-DsRed-Express2(x6)
BlasticidinR
UseTagsExpressionMammalianMutationPromoterNone, ZF6x12(C)_YB_TATA Minimal Promoter, and hEF…Available sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
Plasmid#217509PurposeKD Recombinase dependent conditional 1X ALFA cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
UseTagsALFA tagExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
Plasmid#217511PurposeKD Recombinase dependent conditional smGdP-10XHA cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
UseTagssmGdP-10XHAExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
Plasmid#217510PurposeKD Recombinase dependent conditional smGdP-10XV5 cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
UseTagssmGdP-10XV5ExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
Plasmid#217512PurposeKD Recombinase dependent conditional smGdP-10XOllas cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
UseTagssmGdP-10XOllasExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159676PurposePlasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.DepositorInsertpBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159677PurposePlasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.DepositorInsert[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAT7
Plasmid#204292PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.DepositorInsert3XP3-DsRed
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIPlac204TKC-E2-Crimson-HDEL
Plasmid#38771DepositorInsertE2-Crimson
UseTagsLinker peptide and yeast ER retention signal sequ…ExpressionYeastMutationPromoterTPI1Available sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pE2-Orange-N1
Plasmid#21719DepositorInsertE2-Orange
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTY24
Plasmid#83794PurposeUseful for tagging yeast proteins with C-terminal DsRed tag; G418 selectionDepositorInsertDsRed
UseTagsDsRedExpressionYeastMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pE2-Red/Green-N1
Plasmid#21720DepositorInsertE2-Red/Green
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMJA167
Plasmid#128536PurposeExpresses DsRedDepositorInsertNFAT-DsRed reporter
UseTagsExpressionMammalianMutationPromoterSV40-NFATAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAT10
Plasmid#204293PurposeFor marking CRISPR HDR integrant with DsRed expressed in flight muscles.DepositorInsertMHC-DsRed
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Smad3-shRNA
Plasmid#32806DepositorInsertSmad3 (Smad3 Mouse)
UseRNAiTagsDsRedExpressionMammalianMutationPromoterhU6Available sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJAT30
Plasmid#204294PurposeFor marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.DepositorInsertMHC-DsRed
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only