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Plasmid#226312PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1
UseTagsExpressionBacterialMutationY300APromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB44
Plasmid#226303PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S MR1
UseTagsExpressionBacterialMutationC292S, C310SPromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB43
Plasmid#226302PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertWT CsoS2, frameshift site intact
UseTagsExpressionBacterialMutationPromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_GFPsp
Plasmid#212144PurposePreparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_GFPsp_CamRcargo
Plasmid#212143PurposePreparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCSh2-2300
Plasmid#170984PurposeEncodes sgRNA target 2300 and Kanamycin resistance transposon.DepositorInsertSh-CAST sgRNA 2300
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lyn-YFP-FRB3C
Plasmid#162427Purposefor use in CIT (chemically induced trimerization) systemDepositorInsertLyn-YFP-FRB3C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRB3N-CFP-CAAX
Plasmid#162430Purposefor use in CIT (chemically induced trimerization) systemDepositorInsertFRB3N-CFP-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
YFP-FRB1N-Cb5
Plasmid#162435Purposefor use in CIT (chemically induced trimerization) systemDepositorInsertYFP-FRB1N-Cb5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRB2N-CFP-CAAX
Plasmid#162429Purposefor use in CIT (chemically induced trimerization) systemDepositorInsertFRBN-CFP-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lyn-YFP-FRB2C
Plasmid#162426Purposefor use in CIT (chemically induced trimerization) systemDepositorInsertLyn-YFP-FRB2C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXBK_pFa
Plasmid#114255PurposePOC12397, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type p-aDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXBK_aFb
Plasmid#114256PurposePOC12398, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-bDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXBK_bFc
Plasmid#114257PurposePOC12399, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type b-cDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXBK_aFq
Plasmid#114258PurposePOC12400, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXBK_bFq
Plasmid#114259PurposePOC12401, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type b-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXBK_cFq
Plasmid#114260PurposePOC12402, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type c-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLK_bFc
Plasmid#114231PurposePOC12369, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type b-cDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLK_cFd
Plasmid#114232PurposePOC12370, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type c-dDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLK_dFe
Plasmid#114233PurposePOC12371, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type d-eDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only