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LentiCRISPR v2 sgZER1-1

Plasmid

#191549

Purpose

Expression of spCas9 and sgRNA targetting ZER1

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

spCas9 (ZER1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 sgZER1-2

Plasmid

#191550

Purpose

Expression of spCas9 and sgRNA targetting ZER1

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

spCas9 (ZER1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

Plasmid

#211687

Purpose

U6-sgRNA-EFS-Cas9-P2A-Puro

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF827-recipient_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Plasmid

#211692

Purpose

U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF826-recipient_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211688

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

Plasmid

#172982

Purpose

control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

Plasmid

#86195

Purpose

U6 based expression of N meningitidis sgRNA

Depositor

Scot Wolfe

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

nmCas9 sgRNA cloning cassete

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

Plasmid

#118594

Purpose

Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

sgRNA/Puro-T2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-U6-sgMettl3

Plasmid

#196263

Purpose

Expresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoter

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

Mettl3 sgRNA (Mettl3 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

Plasmid

#139086

Purpose

Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

Plasmid

#139087

Purpose

Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

SpCas9-NG

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-ERCC1 gRNA1

Plasmid

#211630

Purpose

sgRNA-1 against ERCC1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ERCC1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-ERCC6L gRNA2

Plasmid

#211633

Purpose

sgRNA-2 agsinst ERCC6L

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ERCC6L

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-ZWILCH gRNA2

Plasmid

#211635

Purpose

sgRNA-2 against ZWILCH

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ZWILCH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-ZWILCH gRNA1

Plasmid

#211634

Purpose

sgRNA-1 against ZWILCH

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ZWILCH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-ERCC6L gRNA1

Plasmid

#211632

Purpose

sgRNA-1 against ERCC6L

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ERCC6L

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-ERCC1 gRNA2

Plasmid

#211631

Purpose

sgRNA-2 against ERCC1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ERCC1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-CHMP4B gRNA2

Plasmid

#211629

Purpose

sgRNA-2 against CHMP4B

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA CHMP4B

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-CHMP4B gRNA1

Plasmid

#211628

Purpose

sgRNA-1 against CHMP4B

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA CHMP4B

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

Plasmid

#139089

Purpose

Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

xCas9-NG

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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