-
Plasmid#223608PurposeLentiviral expression of human CD80 (Y65A) with SNAP tag in mammalian cellsDepositorInsertCD80 (CD80 Human)
UseLentiviral; mammalian expressionTagsSNAPExpressionMutationY65APromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CLIP-CD28(SGGG)
Plasmid#223607PurposeLentiviral expression of human CD28 (SGGG) with CLIP tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsCLIPExpressionMutationMYPPPY mutated to SGGGPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-dSV40-CD80-mApple
Plasmid#223595PurposeLentiviral expression of human CD80 with mApple in mammalian cellsDepositorInsertCD80 (CD80 Human)
UseLentiviral; mammalian expressionTagsmAppleExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-dSV40-CD80-mGFP
Plasmid#223592PurposeLentiviral expression of human CD80 with mGFP tag in mammalian cellsDepositorInsertCD80 (CD80 Human)
UseLentiviral; mammalian expressionTagsmGFPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SNAP-CD80 (I92R)
Plasmid#223589PurposeLentiviral expression of human CD80 (I92R) with SNAP tag in mammalian cellsDepositorInsertCD80 (CD80 Human)
UseLentiviral; mammalian expressionTagsSNAPExpressionMutationI92RPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-AL-mCh-Cry2WT-NLS
Plasmid#221924PurposeExpression of CBP autoregulatory loop (AL) fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP AL (aa 1529-1607) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the autoregulatory loop (AL) (aa 152…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SUMO-A'-l2-A'-IRES-EmGFP
Plasmid#209125PurposeLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFPDepositorInsertSUMO-A'-l2-A'-IRES-EmGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag29-iCAM-1
Plasmid#205208PurposeThis vector encodes of the synCAM (iCAM1) and GFP nanobody LAG29DepositorInsertsynCAM iCAM1 and Lag29 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag43-iCAM-1
Plasmid#205206PurposeThis vector encodes of the synCAM (iCAM1) and GFP nanobody LAG43DepositorInsertsynCAM iCAM1 and Lag43 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV*/Puro2a-PAG1-GFP
Plasmid#198223PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertPAG1 (PAG1 Human)
UseLentiviralTagsFollowed by EGFP, after a flexible linker and Pre…ExpressionMammalianMutationNonePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-PGL-1-mHP1alpha
Plasmid#181894PurposeSoluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-PGL-1-mHP1alpha (Cbx5 Mustard Weed, Mouse)
UseTagsPHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-nE-mHP1alpha-delCTE
Plasmid#181891PurposeSoluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mustard Weed, Mouse)
UseTagsPHR-mCherryExpressionMammalianMutationS11E, S12E, S13E, S14E, 14 aa C-terminal deletionPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1 (BTLA ITIM)-mGFP
Plasmid#180817PurposeExpression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFPDepositorInsertPD-1 (BTLA ITIM) (PDCD1 Human)
UseLentiviralTagsmEGFPExpressionMutationreplaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…PromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-BTLA (PD-1 ITIM)-mGFP
Plasmid#180818PurposeExpression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFPDepositorInsertBTLA (PD-1 ITIM) (BTLA Human)
UseLentiviralTagsmEGFPExpressionMutationreplaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…PromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-YTH-mCherry-Cry2
Plasmid#177126PurposeExpress YTH domain fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet AssayDepositorInsertYTHDC1 (YTH domain) (YTHDC1 Human)
UseTagsmCherry-CRY2ExpressionMammalianMutationYTH domain onlyPromoterSFFVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-hGCN5-NLS
Plasmid#103827PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorUseTagsExpressionMammalianMutationhGCN5: A566G (E189G), G585T (K195N), G726A (silen…PromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-Emi1_ARHGAP5 UTRs
Plasmid#67927PurposeExpresses mCherry-Emi1 with ARHGAP5 UTRs in mammalian cellsDepositorInsertmCherry-Emi1 (FBXO5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-SIN-PTEN-del(1-25)
Plasmid#30390DepositorInsertPTEN-del(1-25) (PTEN Human)
UseTagsExpressionMammalianMutationdel(1-25)PromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR-SIN-PTEN-del(1-15)
Plasmid#30389DepositorInsertPTEN-del(1-15) (PTEN Human)
UseTagsExpressionMammalianMutationdel(1-15)PromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR-SIN-PTEN-L25A
Plasmid#30388DepositorInsertPTEN-L25A (PTEN Human)
UseTagsExpressionMammalianMutationL25APromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only